Simental-Mendía Mario, Vilchez-Cavazos Félix, García-Garza Rubén, Lara-Arias Jorge, Montes-de-Oca-Luna Roberto, Said-Fernández Salvador, Martínez-Rodríguez Herminia G
Department of Biochemistry and Molecular Medicine, Faculty of Medicine, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, México.
Department of Orthopedics and Traumatology, Faculty of Medicine/University Hospital "José Eleuterio González", Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, México.
Histol Histopathol. 2018 Jun;33(6):609-618. doi: 10.14670/HH-11-961. Epub 2018 Jan 9.
To determine the effects of autologous leukocyte-poor platelet-rich plasma (LP-PRP) on the expression of markers involved in cartilage-extracellular matrix production and inflammation in cartilage explants bearing osteoarthritis.
Cartilage explants and LP-PRP were obtained from 10 patients who underwent total knee arthroplasty. The explants were cultured in spinner flasks for 28 days in the presence of interleukin (IL)-1β and/or LP-PRP. The gene expression of catabolic (MMP13, ADAMTS5, and IL1β) and anabolic factors (COL2A1, ACAN, and SOX9) was quantified. A histological assessment was performed according to a modified Mankin score, and quantification of type II and I collagen deposition.
The gene expression of catabolic factors and the Mankin score were lower in LP-PRP- and LP-PRP/IL-1β- than in IL-1β-treated explants, suggesting less matrix degradation in explants cultured in the presence of LP-PRP. Higher expression of genes involved in cartilage matrix restoration was observed in LP-PRP and LP-PRP/IL-1β- when compared to IL-1β-treated explants. The explants treated with LP-PRP and LP-PRP/IL-1β exhibited a higher deposition of type II collagen as well as a lower deposition of type I collagen and also better surface integrity and a significant increase in the number of chondrocytes.
LP-PRP treatment favored restoration in early osteoarthritic cartilage and reduced the pro-inflammatory effect of IL-1β. LP-PRP is a promising therapy for early osteoarthritis, as it promotes extracellular matrix repair, reduces inflammation, and slows cartilage degeneration.
确定自体贫白细胞富血小板血浆（LP-PRP）对骨关节炎软骨外植体中参与软骨细胞外基质生成和炎症的标志物表达的影响。
从10例行全膝关节置换术的患者中获取软骨外植体和LP-PRP。将外植体在含有白细胞介素（IL）-1β和/或LP-PRP的情况下于旋转培养瓶中培养28天。对分解代谢（MMP13、ADAMTS5和IL1β）和合成代谢因子（COL2A1、ACAN和SOX9）的基因表达进行定量分析。根据改良的曼金评分进行组织学评估，并对II型和I型胶原沉积进行定量分析。
与IL-1β处理的外植体相比，LP-PRP和LP-PRP/IL-1β处理的外植体中分解代谢因子的基因表达和曼金评分更低，这表明在LP-PRP存在下培养的外植体中基质降解较少。与IL-1β处理的外植体相比，LP-PRP和LP-PRP/IL-1β处理的外植体中参与软骨基质修复的基因表达更高。用LP-PRP和LP-PRP/IL-1β处理的外植体表现出更高的II型胶原沉积以及更低的I型胶原沉积，并且表面完整性更好，软骨细胞数量显著增加。
LP-PRP治疗有利于早期骨关节炎软骨的修复，并降低IL-1β的促炎作用。LP-PRP是早期骨关节炎的一种有前景的治疗方法，因为它能促进细胞外基质修复、减轻炎症并减缓软骨退变。
