École Polytechnique de Montréal, Montréal, Québec, Canada.
Institut de Cardiologie de Montréal, Montréal, Québec, Canada.
J Biomed Opt. 2018 Jan;23(1):1-9. doi: 10.1117/1.JBO.23.1.016008.
An automated serial histology setup combining optical coherence tomography (OCT) imaging with vibratome sectioning was used to image eight wild type mouse brains. The datasets resulted in thousands of volumetric tiles resolved at a voxel size of (4.9×4.9×6.5) μm3 stitched back together to give a three-dimensional map of the brain from which a template OCT brain was obtained. To assess deformation caused by tissue sectioning, reconstruction algorithms, and fixation, OCT datasets were compared to both in vivo and ex vivo magnetic resonance imaging (MRI) imaging. The OCT brain template yielded a highly detailed map of the brain structure, with a high contrast in white matter fiber bundles and was highly resemblant to the in vivo MRI template. Brain labeling using the Allen brain framework showed little variation in regional brain volume among imaging modalities with no statistical differences. The high correspondence between the OCT template brain and its in vivo counterpart demonstrates the potential of whole brain histology to validate in vivo imaging.
我们使用一种自动的连续组织学切片方法，将光学相干断层扫描（OCT）成像与振动切片机结合，对 8 只野生型小鼠的大脑进行成像。这些数据集产生了数以千计的体积瓦片，分辨率为（4.9×4.9×6.5）μm3，拼接在一起形成了大脑的三维图谱，从中获得了 OCT 大脑模板。为了评估组织切片、重建算法和固定引起的变形，我们将 OCT 数据集与体内和体外磁共振成像（MRI）进行了比较。OCT 大脑模板生成了高度详细的大脑结构图谱，具有高对比度的白质纤维束，与体内 MRI 模板高度相似。使用 Allen 大脑图谱进行大脑标记，显示不同成像方式之间的脑区体积变化很小，没有统计学差异。OCT 模板脑与其体内对应物之间的高度一致性表明，全脑组织学具有验证体内成像的潜力。
