Jadhav Jagurti, Dutta Sruba, Kale Sandeep, Pratap Amit
a Department of Oils, Oleochemicals and Surfactants Technology , Institute of Chemical Technology, Nathalal Parekh Road , Matunga (East) , Mumbai , India.
b DBT-ICT-CEB , Institute of Chemical Technology , Mumbai , India.
Prep Biochem Biotechnol. 2018 Mar 16;48(3):234-241. doi: 10.1080/10826068.2017.1421967.
Glycolipids are one of the major classes of biosurfactants in which the rhamnolipids are best studied. The present work investigates the optimization of inoculum age and batch time for maximizing the yield of rhamnolipid from Pseudomonas aeruginosa (MTCC 2453). The yield and titer of rhamnolipids were maximum in the fermentation batch with an inoculum age of 24 hr. Batch time studies were performed on biomass production, rhamnolipid production, and sunflower oil utilization. The maximum yield of rhamnolipid was achieved at 96 hr when the culture cells were in the late exponential/early stationary phase. At optimum substrate concentration, maximum yield of 10.8 g/L was achieved. Further, downstream processing of crude rhamnolipid from broth using organic solvent extraction and subsequent purification using adsorption chromatography was done. In this study, chromatographic method was developed for purification of rhamnolipid by adsorption phenomena with more than 88.7% purity and 86.5% recovery. The present study provides new perspective on concepts involving separation by adsorption. Further antimicrobial properties and surfactant properties were studied for rhamnolipid production.
糖脂是生物表面活性剂的主要类别之一，其中对鼠李糖脂的研究最为深入。目前的工作是研究接种龄和分批时间的优化，以最大限度地提高铜绿假单胞菌（MTCC 2453）产生鼠李糖脂的产量。在接种龄为24小时的发酵批次中，鼠李糖脂的产量和效价最高。对生物量生产、鼠李糖脂生产和向日葵油利用进行了分批时间研究。当培养细胞处于指数后期/稳定前期时，在96小时达到了鼠李糖脂的最高产量。在最佳底物浓度下，最高产量达到10.8 g/L。此外，使用有机溶剂萃取从肉汤中对粗鼠李糖脂进行下游加工，并随后使用吸附色谱法进行纯化。在本研究中，开发了通过吸附现象纯化鼠李糖脂的色谱方法，纯度超过88.7%，回收率为86.5%。本研究为涉及吸附分离的概念提供了新的视角。对鼠李糖脂生产的进一步抗菌性能和表面活性剂性能进行了研究。
