de Alencar Almeida Mayara, Conceição Karen Stefany, Teixeira Juliana Perez Marques, Pan Nicole Caldas, Alves Luis Paulo Silveira, Nakazato Gerson, Donatti Lucélia, Souza Emanuel M, Camilios-Neto Doumit, Vignoli Josiane A
Departamento de Bioquímica e Biotecnologia, Universidade Estadual de Londrina, Londrina, PR, Brazil.
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, PR, Brazil.
World J Microbiol Biotechnol. 2025 May 26;41(6):183. doi: 10.1007/s11274-025-04412-9.
Surfactants are amphiphilic molecules with a worldwide billionaire market. Although chemical surfactants are the most available and commonly used, the microbiologically produced congeners have attracted attention due to their low toxicity, biodegradability, and high ecological acceptability. Among microbial surfactants rhamnolipids stand out, with high yields of production, excellent surface-active properties and great potential market. However, the application of these compounds is still limited mostly because of excessive production costs. This study presents a cost-effective process for rhamnolipids production by semi-submerged static-cultivation using agar cubes as substrate. A concentration of 50 g/L of rhamnolipids was reached in semi-submerged static-cultivation using agar cubes containing corn bran and soybean oil. Through this static process, besides avoiding foam, gradual release of nutrients provides a large increase in P. aeruginosa growth and consequently on rhamnolipids production.
表面活性剂是一类具有全球亿万富翁市场的两亲分子。虽然化学表面活性剂是最容易获得且最常用的，但微生物产生的同类物因其低毒性、生物降解性和高生态可接受性而受到关注。在微生物表面活性剂中，鼠李糖脂脱颖而出，具有高产率、优异的表面活性特性和巨大的潜在市场。然而，这些化合物的应用仍然大多受到限制，主要是因为生产成本过高。本研究提出了一种以琼脂块为底物，通过半淹没静态培养生产鼠李糖脂的经济高效方法。在使用含有玉米麸皮和大豆油的琼脂块进行半淹没静态培养时，鼠李糖脂浓度达到了50 g/L。通过这种静态过程，除了避免产生泡沫外，营养物质的逐渐释放还大大促进了铜绿假单胞菌的生长，从而提高了鼠李糖脂的产量。
