State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, P. R China.
Food Funct. 2018 Feb 21;9(2):917-924. doi: 10.1039/c7fo01574g.
Oxidative stress and its correlation with degenerative diseases have been attracting wide attention. In the present study, Lactobacillus plantarum (L. plantarum) CCFM10 and RS15-3 were examined for their abilities to resist oxidative stress in d-galactose (d-gal)-exposed mice. Both strains significantly reversed the changes of hepatic total antioxidant capacity, catalase activity and glutathione content induced by d-gal. The antioxidative abilities of CCFM10 were higher than those of RS15-3. Moreover, the composition of the mice microbiota was changed by d-gal injection, which was characterized by the up-regulated ratio of Firmicutes/Bacteroidetes. At the genus level, the relative abundance of Lactobacillus reduced and the proportion of one genus of Clostridiales increased. However, in probiotic groups, the composition of the microbiota was similar to that of the control group at the phylum and the genus levels, suggesting that probiotic administration can restore the microbiota. Our study suggests that the protective effects of L. plantarum strains on the host microbiota could be one of the mechanisms of their resistance to oxidative stress. Besides this, through comparing the antioxidative capacity of two strains, we also found that the antioxidative capacity of L. plantarum might be strain-specific.
氧化应激及其与退行性疾病的相关性引起了广泛关注。本研究检测了植物乳杆菌（Lactobacillus plantarum）CCFM10 和 RS15-3 抵抗半乳糖（d-gal）暴露小鼠氧化应激的能力。这两种菌株均能显著逆转 d-gal 诱导的肝总抗氧化能力、过氧化氢酶活性和谷胱甘肽含量的变化。CCFM10 的抗氧化能力高于 RS15-3。此外，d-gal 注射会改变小鼠的微生物群组成，其特征是厚壁菌门/拟杆菌门的比例上调。在属水平上，乳杆菌的相对丰度降低，梭菌目（Clostridiales）的一个属的比例增加。然而，在益生菌组中，微生物群的组成在门和属水平上与对照组相似，表明益生菌的给药可以恢复微生物群。我们的研究表明，植物乳杆菌菌株对宿主微生物群的保护作用可能是其抵抗氧化应激的机制之一。此外，通过比较两种菌株的抗氧化能力，我们还发现植物乳杆菌的抗氧化能力可能具有菌株特异性。
