Carton Flavia, Calderan Laura, Malatesta Manuela
University of Verona, Department of Neurological, Biomedical and Movement Sciences.
Eur J Histochem. 2017 Nov 28;61(4):2862. doi: 10.4081/ejh.2017.2862.
Explanted organs and tissues represent suitable experimental systems mimicking the functional and structural complexity of the living organism, with positive ethical and economic impact on research activities. However, their preservation in culture is generally limited, thus hindering their application as experimental models for biomedical research. In the present study, we investigated the potential of an innovative fluid dynamic culture system to improve the structural preservation in vitro of explanted mouse skeletal muscles (soleus). We used light and transmission electron microscopy to compare the morphological features of muscles maintained either in multiwell plates under conventional conditions or in a bioreactor mimicking the flow of physiological fluids. Our results demonstrate that fluid dynamic conditions markedly slowed the progressive structural deterioration of the muscle tissue occurring during the permanence in the culture medium, prolonging the preservation of some organelles such as mitochondria up to 48 h.
移植的器官和组织是模拟生物体功能和结构复杂性的合适实验系统，对研究活动具有积极的伦理和经济影响。然而，它们在培养中的保存通常是有限的，从而阻碍了它们作为生物医学研究实验模型的应用。在本研究中，我们研究了一种创新的流体动力学培养系统改善移植的小鼠骨骼肌（比目鱼肌）体外结构保存的潜力。我们使用光学显微镜和透射电子显微镜比较了在传统条件下多孔板中培养的肌肉与在模拟生理流体流动的生物反应器中培养的肌肉的形态特征。我们的结果表明，流体动力学条件显著减缓了肌肉组织在培养基中留存期间发生的渐进性结构退化，将一些细胞器（如线粒体）的保存时间延长至48小时。
