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一种用于研究透明质酸给药对人皮肤外植体中胶原蛋白组织影响的流体动力学体外系统。

A Fluid Dynamic In Vitro System to Study the Effect of Hyaluronic Acid Administration on Collagen Organization in Human Skin Explants.

作者信息

Galvan Andrea, Lacavalla Maria Assunta, Boschi Federico, Cisterna Barbara, Dalla Pozza Edoardo, Vigato Enrico, Carton Flavia, Malatesta Manuela, Calderan Laura

机构信息

Department of Neurosciences, Biomedicine and Movement Sciences, University of Verona, 37134 Verona, Italy.

Department of Diagnostic and Public Health, University of Verona, 37134 Verona, Italy.

出版信息

Int J Mol Sci. 2025 Jun 4;26(11):5397. doi: 10.3390/ijms26115397.

DOI:10.3390/ijms26115397
PMID:40508206
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12154972/
Abstract

Hyaluronic acid (HA) is an unbranched polysaccharide particularly abundant in the extracellular matrix (ECM) of soft connective tissues. In humans, about 50% of the total HA in the organism is localized in the skin. HA plays an essential role in the hydration of the ECM, in the regulation of tissue homeostasis, in the resistance to mechanical stimuli/forces, and in the modulation of tissue regeneration. For these reasons, HA is widely used in regenerative medicine and cosmetics. In this study we used an innovative fluid dynamic system to investigate the effects of a cross-linked macrostructural HA formulation on dermal collagen of healthy human skin explants. The good preservation of skin explants provided by the bioreactor allowed applying refined high-resolution microscopy techniques to analyze in situ the HA-induced modifications on the ECM collagen fibrils up to 48 h from the application on the skin surface. Results demonstrated that this HA formulation, commercially proposed for subcutaneous injection, may act on dermal ECM also when applied transcutaneously, improving ECM hydration and modifying the organization of the collagen fibrils. These findings, obtained by the original combination of explanted human skin use with an advanced culture system and multiscale imaging techniques, are consistent with the volumizing and anti-aging effect of HA.

摘要

透明质酸（HA）是一种无分支的多糖，在软结缔组织的细胞外基质（ECM）中含量尤为丰富。在人类机体中，约50%的总HA存在于皮肤中。HA在ECM的水合作用、组织稳态的调节、对机械刺激/力的抵抗以及组织再生的调节中发挥着重要作用。基于这些原因，HA被广泛应用于再生医学和化妆品领域。在本研究中，我们使用了一种创新的流体动力学系统，来研究一种交联的宏观结构HA制剂对健康人皮肤外植体真皮胶原蛋白的影响。生物反应器对皮肤外植体的良好保存，使得我们能够应用精细的高分辨率显微镜技术，在将HA制剂应用于皮肤表面后的48小时内，原位分析HA对ECM胶原纤维的诱导性修饰。结果表明，这种市售的用于皮下注射的HA制剂，经皮应用时也可作用于真皮ECM，改善ECM水合作用并改变胶原纤维的组织结构。这些通过将人皮肤外植体与先进培养系统及多尺度成像技术进行创新性结合而获得的研究结果，与HA的填充和抗老化作用相符。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeec/12154972/73edbfbb7c9d/ijms-26-05397-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeec/12154972/169c933d9f3c/ijms-26-05397-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeec/12154972/094ea9efd104/ijms-26-05397-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeec/12154972/73edbfbb7c9d/ijms-26-05397-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeec/12154972/169c933d9f3c/ijms-26-05397-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeec/12154972/ec98009e61f1/ijms-26-05397-g002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeec/12154972/0d7441d60983/ijms-26-05397-g006.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aeec/12154972/73edbfbb7c9d/ijms-26-05397-g008.jpg

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