Herrera M, Herrera J M, Cantatore S, Aguilar J, Felipe A, Fumuso E
Facultad de Ciencias Veterinarias, Laboratorio de Clínica y Reproducción Equina, CIVETAN, UNICEN, Tandil, Argentina.
Facultad de Ciencias Veterinarias, Área de Ciencias Morfológicas, UNICEN, Tandil, Argentina.
Anat Histol Embryol. 2018 Apr;47(2):153-158. doi: 10.1111/ahe.12335. Epub 2018 Jan 3.
Uterine acute post-breeding inflammation is a physiological tissue response to the entry of exogenous elements, with persistent endometritis being the main pathology responsible for subfertility in the mare (Equus ferus caballus; Linnaeus, 1758). Mares can be classified as susceptible or resistant to endometritis according to their ability to remove intrauterine fluid within 48 hr after experimental inoculation. Endometrial biopsy is a technique that is commonly used to establish the degree of lesions that can affect the fertility of the mare. Endometrial histomorphometry is an objective and highly precise diagnostic method. The aim of this study was to compare, during oestrus, the endometrial histomorphometry of mares previously classified as susceptible (SM) or resistant (RM) to endometritis. Endometrial biopsies from 24 mares at the oestrus phase of the cycle were obtained. For the histomorphometric analysis, samples were histologically processed and subjected to routine Haematoxylin-Eosin staining. For the evaluation, the variables were considered as follows: 1-Height of the lining and glandular epithelia (Lining SM = 15.9 μm vs. RM = 13.3 μm; Glandular SM = 15.0 μm vs. RM = 13.0 μm); 2-Perpendicular diameters of endometrial glands (SM = 51.3 μm vs. RM = 44.8 μm); 3-Number of endometrial glands per field (SM = 24.8 glands/field vs. RM = 20.5 glands/field). The results from this study suggest the existence of a relationship between the studied characteristics and the susceptibility/resistance to post-breeding endometritis in mares. Thus, increased epithelial height, greater glandular density and greater development of the glands during oestrus would be related to a higher susceptibility to endometritis.
子宫配种后急性炎症是机体对外源性物质侵入的一种生理性组织反应，持续性子宫内膜炎是母马（Equus ferus caballus；林奈，1758）繁殖力低下的主要病理原因。根据母马在实验接种后48小时内清除子宫内液体的能力，可将其分为易患子宫内膜炎或对子宫内膜炎有抵抗力。子宫内膜活检是一种常用的技术，用于确定可能影响母马繁殖力的病变程度。子宫内膜组织形态计量学是一种客观且高度精确的诊断方法。本研究的目的是在发情期比较先前被分类为易患（SM）或抵抗（RM）子宫内膜炎的母马的子宫内膜组织形态计量学。在发情周期阶段从24匹母马获取子宫内膜活检样本。对于组织形态计量分析，样本进行组织学处理并进行常规苏木精-伊红染色。为进行评估，变量如下：1-衬里和腺上皮高度（衬里SM = 15.9μm对RM = 13.3μm；腺SM = 15.0μm对RM = 13.0μm）；2-子宫内膜腺的垂直直径（SM = 51.3μm对RM = 44.8μm）；3-每视野子宫内膜腺数量（SM = 24.8个腺/视野对RM = 20.5个腺/视野）。本研究结果表明所研究的特征与母马配种后子宫内膜炎的易感性/抵抗力之间存在关联。因此，发情期上皮高度增加、腺密度增加和腺发育程度更高与子宫内膜炎易感性更高有关。
