Departamento de Química, , Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo 3900, Brasil.
Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, 3900, Brasil.
J Biomed Mater Res B Appl Biomater. 2018 Oct;106(7):2524-2534. doi: 10.1002/jbm.b.34069. Epub 2018 Jan 4.
Calcium phosphates (CaPs) are biomaterials widely used in tissue regeneration with outstanding biological performance. Although the tremendous improvements achieved in CaP's materials research over the years, their interaction with physiological environments still need to be fully understood. The aim of this study is to explore a biomimetic Langmuir-Blodgett (LB) membrane to template the growth of hydroxyapatite (HAp) coatings on Ti surfaces and the ability of these coatings in inducing biomineralization by osteoblasts cultured in vitro. Changing the phospholipids (i.e., dihexadecyl phosphate (DHP) or octadecylphosphonic acid (OPA)), we also tuned the surface Ca concentration. This structural feature gave rise to different LB-hybrid surfaces where the concentration of Ca in the OPA/HAp was higher than the concentration of Ca in DHP/HAp coating. The higher Ca amount on OPA/HAp coatings, allied to the physical-chemical features, lead to different responses on osteoblasts, stimulating or inhibiting the natural biomineralization. The OPA/HAp coating caused a delay in the osteoblast proliferation as indicated by the decrease in the cell viability at the 7th culture day. Improved cell differentiation triggered by the DHP/HAp coating resulted in higher osteoblast biomineralization. The present data underscore that besides both coatings being composed by HAp, the final interfacial composition and physical-chemical properties influence differently the osteoblast behavior. Although the best osteoblast's viability was found to OPA/HAp, our dataset attested that DHP/HAp induced mineralization more effectively than that. This unexpected finding highlight the importance of deeply understanding the biomaterial interface and suggest a promising approach to the design of biofunctional LB-based coatings with tunable properties. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2524-2534, 2018.
钙磷酸盐（CaPs）是一种广泛应用于组织再生的生物材料，具有出色的生物性能。尽管近年来在 CaP 材料研究方面取得了巨大的进展，但它们与生理环境的相互作用仍需要充分理解。本研究旨在探索一种仿生 Langmuir-Blodgett（LB）膜，以模板化在 Ti 表面生长羟基磷灰石（HAp）涂层，并研究这些涂层在体外培养的成骨细胞中诱导生物矿化的能力。通过改变磷脂（即二十六烷基磷酸酯（DHP）或十八烷基膦酸（OPA）），我们还可以调整表面 Ca 浓度。这种结构特征导致了不同的 LB-杂化表面，其中 OPA/HAp 中的 Ca 浓度高于 DHP/HAp 涂层中的 Ca 浓度。OPA/HAp 涂层中较高的 Ca 量，加上物理化学特性，导致了成骨细胞的不同反应，刺激或抑制了自然矿化。OPA/HAp 涂层导致成骨细胞增殖延迟，第 7 天培养时细胞活力下降。DHP/HAp 涂层引发的细胞分化改善导致成骨细胞生物矿化增加。本数据强调，尽管两种涂层均由 HAp 组成，但最终界面组成和物理化学性质会对成骨细胞行为产生不同的影响。虽然发现 OPA/HAp 具有最佳的成骨细胞活力，但我们的数据表明 DHP/HAp 诱导矿化的效果优于 OPA/HAp。这一意外发现强调了深入理解生物材料界面的重要性，并为设计具有可调特性的基于 LB 的生物功能涂层提供了有前途的方法。 © 2018 Wiley Periodicals, Inc. J 生物材料研究杂志 B：应用生物材料，106B：2524-2534，2018。
