Department of Dental Technology and Materials Science, Central Taiwan University of Science and Technology, Beitun District, Taichung City 40601, Taiwan, ROC.
Acta Biomater. 2011 Nov;7(11):4081-7. doi: 10.1016/j.actbio.2011.07.004. Epub 2011 Jul 13.
This study attempts to enhance the osseointegration of titanium implants by adopting a micro-arc treatment (MAT) capable of replacing calcium (Ca) with different percentages of strontium (Sr) in order to fabricate strontium-containing hydroxyapatite (Sr-HAp) coatings. Sr, regarded as a significant therapy promoting bone mass and bone strength, has a dual mechanism, enhancing osteoblast differentiation and inhibiting osteoclast differentiation. This study also investigates how Sr content affects the microstructure of and osteoblast/osteoclast growth on the coatings. Experimental results indicate that an increase in the Sr content in the electrolyte bath results in a greater degree of Sr substitution at Ca sites within the HAp phase, facilitating the formation of Sr-HAp coatings with Sr fully solid soluble in the HAp phase. Irrespective of the Sr content, most coatings are similar in porous morphology and pore size. Additionally, the Sr-HAp coating shows higher osteoblast compatibility than raw titanium metal and the HAp coating. Moreover, cell adhesion and proliferation after 48 h was greater than that after 4 h, indicating that Sr can stimulate osteoblast adhesion and proliferation. Further, Sr significantly inhibits osteoclast differentiation when the Sr-HAp coatings exceed 38.9 at.% Sr.
本研究试图通过采用微弧处理（MAT）来增强钛植入物的骨整合能力，该处理能够用不同百分比的锶（Sr）替代钙（Ca），从而制备含锶的羟基磷灰石（Sr-HAp）涂层。Sr 被认为是一种促进骨量和骨强度的重要治疗方法，具有双重机制，既能促进成骨细胞分化，又能抑制破骨细胞分化。本研究还探讨了 Sr 含量如何影响涂层的微观结构和成骨细胞/破骨细胞的生长。实验结果表明，电解液中 Sr 含量的增加会导致 HAp 相中更多的 Ca 位被 Sr 取代，从而有利于形成 Sr 完全固溶在 HAp 相中的 Sr-HAp 涂层。无论 Sr 含量如何，大多数涂层的多孔形貌和孔径都相似。此外，Sr-HAp 涂层比原始钛金属和 HAp 涂层具有更高的成骨细胞相容性。此外，细胞在 48 h 时的黏附和增殖大于 4 h 时的黏附和增殖，表明 Sr 可以刺激成骨细胞的黏附和增殖。进一步的研究表明，当 Sr-HAp 涂层中 Sr 的含量超过 38.9 at.%时，它可以显著抑制破骨细胞的分化。
