Fisheries College, Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Jimei University, Xiamen, Fujian, China.
State Key Laboratory of Large Yellow Croaker Breeding, Ningde, Fujian, China.
Microbiologyopen. 2018 Jun;7(3):e00568. doi: 10.1002/mbo3.568. Epub 2018 Jan 3.
Vibrio aestuarianus is an opportunistic environmental pathogen that has been associated with epidemics in cultured shrimp Penaeus vannamei. Hepatopancreas microsporidian (HPM) and monodon slow growth syndrome (MSGS) have been reported in cultured P. vannamei. In this study, we sequenced and assembled the whole genome of V. aestuarianus strain W-40, a strain that was originally isolated from the intestines of an infected P. vannamei. The genome of V. aestuarianus strain W-40 contains two circular chromosomes of 483,7307 bp with a 46.23% GC content. We identified 4,457 open reading frames (ORFs) that occupy 86.35% of the genome. Vibrio aestuarianus strain W-40 consists primarily of the ATP-binding cassette (ABC) transporter system and the phosphotransferase system (PTS). CagA is a metabolism system that includes bacterial extracellular solute-binding protein. Glutathione reductase can purge superoxide radicals (O22-) and hydrogen peroxide (H O ) damage in V. aestuarianus strain W-40. The presence of two compete type I restriction-modification systems was confirmed. A total of 42 insertion sequences (IS) elements and 16 IS elements were identified. Our results revealed a host of virulence factors that likely contribute to the pathogenicity of V. aestuarianus strain W-40, including the virulence factor genes vacA, clpC, and bvgA, which are important for biofilm dispersion. Several bacitracin and tetracycline antibiotic resistance-encoding genes and type VI secretion systems were also identified in the genome. The complete genome sequence will aid future studies of the pathogenesis of V. aestuarianus strain W-40 and allow for new strategies to control disease to be developed.
韦氏气单胞菌是一种机会性环境病原体，与养殖南美白对虾的流行病有关。已报道在养殖的凡纳滨对虾中存在肝胰腺微孢子虫（HPM）和单性慢生长综合征（MSGS）。在本研究中，我们对最初从感染的南美白对虾肠道中分离到的韦氏气单胞菌 W-40 菌株进行了全基因组测序和组装。韦氏气单胞菌 W-40 基因组包含两条大小为 4837307 bp 的圆形染色体，GC 含量为 46.23%。我们鉴定出 4457 个开放阅读框（ORFs），占基因组的 86.35%。韦氏气单胞菌 W-40 主要由三磷酸腺苷结合盒（ABC）转运系统和磷酸转移酶系统（PTS）组成。CagA 是一个代谢系统，包括细菌细胞外溶质结合蛋白。谷胱甘肽还原酶可以清除超氧自由基（O22-）和过氧化氢（H2O2）对韦氏气单胞菌 W-40 的损伤。证实存在两种竞争型 I 型限制修饰系统。总共鉴定出 42 个插入序列（IS）元件和 16 个 IS 元件。我们的研究结果揭示了许多毒力因子，这些毒力因子可能导致韦氏气单胞菌 W-40 的致病性，包括毒力因子基因 vacA、clpC 和 bvgA，这些基因对于生物膜的分散很重要。在基因组中还鉴定到几种杆菌肽和四环素抗生素耐药基因和 VI 型分泌系统。完整的基因组序列将有助于未来研究韦氏气单胞菌 W-40 的发病机制，并为开发新的疾病控制策略提供依据。
