Lovett D L, Felder D L
Biol Bull. 1990 Apr;178(2):144-159. doi: 10.2307/1541973.
Whole specimens of developmental stages of Penaeus setiferus (Linnaeus, 1767) were homogenized and assayed for activities of digestive enzymes. In all developmental stages, activities were present for trypsin, carboxypeptidase A and B, amylase, and non-specific esterase; none for pepsin or lipase were detected. Activities assayed with substrates for chymotrypsin and aminopeptidase are not apparently due to the presence of these enzymes in the gut. Peak activities for all enzymes occurred during late zoeal or early mysis larval stages; low activities occurred at metamorphosis. During postlarval development, amylase activity increased steadily (by a tenfold increase over five weeks), whereas most other enzyme activities were relatively constant until the fifth week of postlarval development. Although it alters enzyme activity, diet does not appear to be the primary effector of ontogenetic change in digestive enzyme activity. Instead, ontogenetic change in digestive enzyme activity may reflect either a developmentally cued change in enzyme synthesis, or a secondary effect of change in the function and relative size of the midgut during its differentiation.
对凡纳滨对虾（林奈，1767年）发育阶段的完整样本进行匀浆，并测定其消化酶活性。在所有发育阶段，均检测到胰蛋白酶、羧肽酶A和B、淀粉酶以及非特异性酯酶的活性；未检测到胃蛋白酶或脂肪酶的活性。用胰凝乳蛋白酶和氨肽酶底物测定的活性显然不是由于肠道中存在这些酶。所有酶的活性峰值出现在后期溞状幼体或早期糠虾幼体阶段；变态时活性较低。在幼体后期发育过程中，淀粉酶活性稳步增加（在五周内增加了十倍），而大多数其他酶活性在幼体后期发育的第五周之前相对恒定。尽管饮食会改变酶活性，但它似乎不是消化酶活性个体发育变化的主要影响因素。相反，消化酶活性的个体发育变化可能反映了酶合成中发育线索引导的变化，或者是中肠在分化过程中功能和相对大小变化的次要影响。
