Horie K
Nihon Geka Gakkai Zasshi. 1985 Jul;86(7):787-801.
In order to establish passive cancer immunotherapy by in vitro culture of lymphocytes, several methods were devised to induce and augment anti-tumor activity of peripheral blood lymphocytes (PBL) in the presence of interleukin-2 (IL-2). In vitro experiment: Weak anti-tumor activity appeared on culture of PBL in the presence of IL-2. On addition of MMC treated tumor antigen, the anti-tumor activity was augmented. Anti-tumor activity further increased on addition of peritoneal exudate macrophages or peripheral whole white blood cells containing monocytes to (2) as accessory cells. Effect similar to (3) was obtained in MLTC of peripheral whole white blood cells. [II] In Winn neutralization test, the strongest tumor-neutralizing activity was seen in the group of cultured lymphocytes with antigen and in the group of cultured leukocytes with antigen. This result agreed excellently with the results of the in vitro experiments [I]. [III] In the therapeutic experiment: Significantly better effect was noted in the treated group by cultured leukocytes with antigen than in the treated group by non-cultured lymphocytes. The treatment with 10(7) lymphocytes produced definitely prolonged survival of tumor-bearing rats compared to the treatment of 10(6) or less number lymphocytes.
为了通过淋巴细胞的体外培养建立被动癌症免疫疗法，人们设计了几种方法来在白细胞介素-2（IL-2）存在的情况下诱导和增强外周血淋巴细胞（PBL）的抗肿瘤活性。体外实验：在IL-2存在的情况下培养PBL时出现较弱的抗肿瘤活性。加入丝裂霉素C处理的肿瘤抗原后，抗肿瘤活性增强。将腹腔渗出巨噬细胞或含有单核细胞的外周全白细胞作为辅助细胞加入到（2）中，抗肿瘤活性进一步增加。在外周全白细胞的混合淋巴细胞肿瘤培养（MLTC）中获得了与（3）相似的效果。[II]在温氏中和试验中，在含有抗原的培养淋巴细胞组和含有抗原的培养白细胞组中观察到最强的肿瘤中和活性。该结果与体外实验[I]的结果非常吻合。[III]在治疗实验中：用含有抗原的培养白细胞治疗的组比用未培养的淋巴细胞治疗的组效果明显更好。与用10⁶或更少数量淋巴细胞治疗相比，用10⁷个淋巴细胞治疗能显著延长荷瘤大鼠的生存期。
