Horie K, Yamasaki T, Nishimura K, Tsuchiya S, Yoshizawa A, Akimoto R, Watanabe H, Shirasawa K, Suzuki M, Tsujimoto Y
Gan To Kagaku Ryoho. 1984 Jun;11(6):1269-75.
This basic study was performed to determine whether the anti-tumor effect of lymphocytes obtained from the peripheral blood of a tumor bearing host can be increased when cultured in vitro with Mitomycin C (MMC)-treated AH109A tumor cells, using interleukin-2 (IL-2). Donryu rats were used as tumor bearing hosts. The following results were obtained. Weak anti-tumor activity of lymphocytes was noted 7 days after lymphocytes were cultured in the medium to which only IL-2 was added. Anti-tumor activity was augmented by adding antigen (MMC treated tumor cells) to the above (1). Anti-tumor activity was further augmented by adding to (2) above antigen presenting cells such as intraperitoneal exudate macrophages or peripheral hole leukocytes. Anti-tumor activity of lymphocytes was detected when IL-2 and MMC treated antigen were added to the peripheral hole leukocytes containing the lymphocytes.
进行这项基础研究是为了确定，当从荷瘤宿主外周血获取的淋巴细胞与经丝裂霉素C（MMC）处理的AH109A肿瘤细胞在体外使用白细胞介素-2（IL-2）进行培养时，其抗肿瘤作用是否能够增强。选用Donryu大鼠作为荷瘤宿主。获得了以下结果。在仅添加IL-2的培养基中培养淋巴细胞7天后，观察到淋巴细胞的抗肿瘤活性较弱。通过向上述（1）中添加抗原（经MMC处理的肿瘤细胞），抗肿瘤活性增强。通过向上述（2）中添加诸如腹腔渗出巨噬细胞或外周孔白细胞等抗原呈递细胞，抗肿瘤活性进一步增强。当将IL-2和经MMC处理的抗原添加到含有淋巴细胞的外周孔白细胞中时，检测到淋巴细胞的抗肿瘤活性。
