Department of Pathology and Infectious Diseases, School of Veterinary Medicine, University of Surrey, Guildford, UK.
CBR Division, Defence Science & Technology Laboratory, Salisbury, UK.
Int J Exp Pathol. 2017 Dec;98(6):347-355. doi: 10.1111/iep.12258. Epub 2018 Jan 7.
Organ tissue damage is a key contributor to host morbidity and mortality following infection with microbial agents. Severe immune responses, excessive cellular recruitment and necrosis of cells all play a role in disease pathology. Understanding the pathogenesis of disease can aid in identifying potential new therapeutic targets or simply act as a diagnostic tool. Burkholderia pseudomallei is a Gram-negative bacterium that can cause acute and chronic diseases. The BALB/c mouse has been shown to be highly susceptible to aerosol challenge with B. pseudomallei and hence acts as a good model to study the acute and potentially lethal form of the disease melioidosis. In our study, BALB/c mice were challenged and culled at predetermined time points to generate a pathological time course of infection. Lung, liver and spleen were subjected to pathological and immunohistochemical analysis. The number and type of microscopic lesions within each organ, as well as the location and the mean percentage of neutrophils, B cells, T cells and Burkholderia capsule antigen within the lesions, were all characterized during the time course. Neutrophils were determined as the key player in tissue pathology and generation of lesions, with B cells playing an insignificant role. This detailed pathological assessment increases our understanding of B. pseudomallei disease.
组织损伤是宿主感染微生物后发病和死亡的关键因素。严重的免疫反应、过度的细胞募集和细胞坏死都在疾病发病机制中起作用。了解疾病的发病机制有助于确定潜在的新治疗靶点，或者仅作为诊断工具。类鼻疽伯克霍尔德菌是一种革兰氏阴性细菌，可引起急性和慢性疾病。BALB/c 小鼠对气溶胶感染类鼻疽伯克霍尔德菌非常敏感，因此是研究急性和潜在致命形式疾病类鼻疽病的良好模型。在我们的研究中，BALB/c 小鼠在预定时间点受到挑战并被处死，以产生感染的病理时间过程。对肺、肝和脾进行病理和免疫组织化学分析。在整个时间过程中，对每个器官内的微观病变的数量和类型、病变的位置以及中性粒细胞、B 细胞、T 细胞和伯克霍尔德菌荚膜抗原的平均百分比进行了描述。中性粒细胞被确定为组织病理学和病变产生的关键因素，而 B 细胞的作用微不足道。这种详细的病理评估增加了我们对类鼻疽伯克霍尔德菌病的理解。
