Department of Dental Pharmacology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
Department of Clinical Oral Oncology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
Oral Dis. 2018 Jul;24(5):741-748. doi: 10.1111/odi.12826. Epub 2018 Apr 17.
Transcription factor EB (TFEB) is a master regulator of lysosomal biogenesis and plays an important role in various cancers. However, the function of TFEB in oral squamous cell carcinomas has not been examined. The aim of this study was to elucidate the role of TFEB in oral squamous cell carcinomas.
Expression levels of TFEB were examined in six different human oral squamous carcinoma cells: HSC2, HSC3, HSC4, SAS, OSC20, and SCC25. Knockdown of TFEB using small interfering RNA in HSC2 and HSC4 cells was performed. Cell morphology was observed by immunofluorescence microscopy. Cell proliferation, invasion, and adhesion were analyzed.
Expression levels of TFEB were high in HSC2, moderate in HSC4 and SCC25, and low in HSC3 and OSC20 cells. Knockdown of TFEB did not affect proliferation of HSC2 and HSC4 cells, but did induced enlargement of lysosomes and endosomes in HSC4 cells. TFEB silencing reduced invasion and migration of these HSC cell squamous carcinoma cells; however, increased cell adhesion was also observed.
TFEB knockdown reduces invasion and migration of cancer cells, likely through lysosomal regulation. Taken together, TFEB influences cell invasion and migration of oral squamous cell carcinomas.
转录因子 EB（TFEB）是溶酶体生物发生的主要调节因子，在各种癌症中发挥重要作用。然而，TFEB 在口腔鳞状细胞癌中的功能尚未被研究。本研究旨在阐明 TFEB 在口腔鳞状细胞癌中的作用。
在六种不同的人口腔鳞状癌细胞：HSC2、HSC3、HSC4、SAS、OSC20 和 SCC25 中检查了 TFEB 的表达水平。使用小干扰 RNA 对 HSC2 和 HSC4 细胞中的 TFEB 进行敲低。通过免疫荧光显微镜观察细胞形态。分析细胞增殖、侵袭和黏附。
TFEB 的表达水平在 HSC2 中较高，在 HSC4 和 SCC25 中中等，在 HSC3 和 OSC20 细胞中较低。TFEB 的敲低并不影响 HSC2 和 HSC4 细胞的增殖，但在 HSC4 细胞中诱导了溶酶体和内体的增大。TFEB 沉默减少了这些 HSC 细胞鳞状癌细胞的侵袭和迁移；然而，也观察到细胞黏附增加。
TFEB 敲低减少了癌细胞的侵袭和迁移，可能通过溶酶体调节。综上所述，TFEB 影响口腔鳞状细胞癌的细胞侵袭和迁移。
