Krieter Detlef H, Lange Florian, Lemke Horst-Dieter, Bonn Florian, Wanner Christoph
Department of Medicine I, Division of Nephrology, University Hospital Würzburg, Würzburg, Germany.
eXcorLab GmbH, Obernburg, Germany.
Ther Apher Dial. 2018 Apr;22(2):189-195. doi: 10.1111/1744-9987.12649. Epub 2018 Jan 9.
Technical problems during clinical lipid apheresis interfere with fractionator performance. Therefore, a large animal model was established to characterize a new plasma fractionation membrane. Four sheep were randomized, controlled, and crossover subjected to double ofiltration lipoprotein apheresis with three specimens of FractioPES having slightly different HDL sieving coefficients (S ) (FPESa, 0.30, FPESb, 0.26, and FPESc, 0.22) versus a control fractionator (EVAL). S and reduction ratios were determined for LDL, HDL, fibrinogen, IgG, and albumin. Compared to EVAL (0.42 ± 0.04 to 0.74 ± 0.08) and FPESa (0.36 ± 0.06 to 0.64 ± 0.04), S for HDL were lower (P < 0.05) with FPESc (0.30 ± 0.04 to 0.49 ± 0.10). Fibrinogen S were higher (P < 0.05) with EVAL (0.02 ± 0.01 to 0.40 ± 0.08) compared to FPESb (0.05 ± 0.02 to 0.26 ± 0.34) and FPESc (0.01 ± 0.01 to 0.21 ± 0.16). No further differences were determined. The animal model distinguished between minor differences in fractionation membrane permeability, demonstrating equivalent sieving of FPESa and EVAL and slightly inferior permeability of FPESb and FPESc.
临床血脂分离术中的技术问题会影响分离器的性能。因此，建立了一个大型动物模型来表征一种新型血浆分离膜。将四只绵羊随机分组、对照并进行交叉实验，使其接受双重过滤脂蛋白分离术，使用三种具有略有不同高密度脂蛋白筛分系数（S）的FractioPES样本（FPESa，0.30；FPESb，0.26；FPESc，0.22），并与对照分离器（EVAL）进行比较。测定了低密度脂蛋白、高密度脂蛋白、纤维蛋白原、免疫球蛋白G和白蛋白的S及降低率。与EVAL（0.42±0.04至0.74±0.08）和FPESa（0.36±0.06至0.64±0.04）相比，FPESc（0.30±0.04至0.49±0.10）的高密度脂蛋白S较低（P<0.05）。与FPESb（0.05±0.02至0.26±0.34）和FPESc（0.01±0.01至0.21±0.16）相比，EVAL（0.02±0.01至0.40±0.08）的纤维蛋白原S较高（P<0.05）。未发现其他差异。该动物模型区分了分离膜通透性的微小差异，表明FPESa和EVAL的筛分效果相当，而FPESb和FPESc的通透性略差。
