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通过机械活化和金属盐协同预处理克服生物质顽固性以增强木质纤维素的酶促转化

Overcoming biomass recalcitrance by synergistic pretreatment of mechanical activation and metal salt for enhancing enzymatic conversion of lignocellulose.

作者信息

Zhang Yanjuan, Huang Min, Su Jianmei, Hu Huayu, Yang Mei, Huang Zuqiang, Chen Dong, Wu Juan, Feng Zhenfei

机构信息

1School of Chemistry and Chemical Engineering, Guangxi University, Nanning, 530004 China.

2State Key Laboratory of Non-Food Biomass and Enzyme Technology, Guangxi Academy of Sciences, Nanning, 530007 China.

出版信息

Biotechnol Biofuels. 2019 Jan 10;12:12. doi: 10.1186/s13068-019-1354-6. eCollection 2019.

DOI:10.1186/s13068-019-1354-6
PMID:30647772
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6327530/
Abstract

BACKGROUND

Due to biomass recalcitrance, including complexity of lignocellulosic matrix, crystallinity of cellulose, and inhibition of lignin, the bioconversion of lignocellulosic biomass is difficult and inefficient. The aim of this study is to investigate an effective and green pretreatment method for overcoming biomass recalcitrance of lignocellulose.

RESULTS

An effective mechanical activation (MA) + metal salt (MAMS) technology was applied to pretreat sugarcane bagasse (SCB), a typical kind of lignocellulosic biomass, in a stirring ball mill. Chlorides and nitrates of Al and Fe showed better synergistic effect with MA, especially AlCl, ascribing to the interaction between metal salt and oxygen-containing groups induced by MA. Comparative studies showed that MAMS pretreatment effectively changed the recalcitrant structural characteristics of lignocellulosic matrix and reduced the inhibitory action of lignin on enzymatic conversion of SCB. The increase in hydroxyl and carboxyl groups of lignin induced by MAMS pretreatment led to the increase of its hydrophilicity, which could weaken the binding force between cellulase and lignin and reduce the nonproductive binding of cellulase enzymes to lignin.

CONCLUSIONS

MAMS pretreatment significantly enhanced the enzymatic digestibility of polysaccharides substrate by overcoming biomass recalcitrance without the removal of lignin from enzymatic hydrolysis system.

摘要

背景

由于生物质的顽固性，包括木质纤维素基质的复杂性、纤维素的结晶度以及木质素的抑制作用，木质纤维素生物质的生物转化困难且效率低下。本研究的目的是研究一种有效且绿色的预处理方法，以克服木质纤维素的生物质顽固性。

结果

采用有效的机械活化（MA）+金属盐（MAMS）技术在搅拌球磨机中对典型的木质纤维素生物质甘蔗渣（SCB）进行预处理。铝和铁的氯化物和硝酸盐与MA表现出更好的协同效应，尤其是AlCl，这归因于MA诱导的金属盐与含氧基团之间的相互作用。对比研究表明，MAMS预处理有效地改变了木质纤维素基质的顽固结构特征，并降低了木质素对SCB酶促转化的抑制作用。MAMS预处理诱导木质素中羟基和羧基的增加导致其亲水性增加，这可以削弱纤维素酶与木质素之间的结合力，并减少纤维素酶与木质素的非生产性结合。

结论

MAMS预处理通过克服生物质顽固性显著提高了多糖底物的酶解消化率，而无需从酶解系统中去除木质素。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd71/6327530/d997b9cca8f3/13068_2019_1354_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd71/6327530/3ec86bd1a5b2/13068_2019_1354_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd71/6327530/3ce7d3d6f628/13068_2019_1354_Fig2_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd71/6327530/71af3ad8c09b/13068_2019_1354_Fig4_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd71/6327530/0f58177c80ed/13068_2019_1354_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd71/6327530/cb80eed8984c/13068_2019_1354_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd71/6327530/d997b9cca8f3/13068_2019_1354_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd71/6327530/3ec86bd1a5b2/13068_2019_1354_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd71/6327530/3ce7d3d6f628/13068_2019_1354_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd71/6327530/1d34442bf4d5/13068_2019_1354_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd71/6327530/71af3ad8c09b/13068_2019_1354_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd71/6327530/80512d1df4ef/13068_2019_1354_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd71/6327530/0f58177c80ed/13068_2019_1354_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd71/6327530/cb80eed8984c/13068_2019_1354_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd71/6327530/d997b9cca8f3/13068_2019_1354_Fig8_HTML.jpg

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