基质金属蛋白酶-1（-519A/G、-1607 1G/2G）、基质金属蛋白酶-3（Lys45Glu）、基质金属蛋白酶-7（-181A/G）和基质金属蛋白酶-12（-82A/G）变体及血浆 MMP 水平与突尼斯人群肥胖相关表型和微血管反应的关系。
Role of MMP-1 (-519A/G, -1607 1G/2G), MMP-3 (Lys45Glu), MMP-7 (-181A/G), and MMP-12 (-82A/G) Variants and Plasma MMP Levels on Obesity-Related Phenotypes and Microvascular Reactivity in a Tunisian Population.
机构信息
Unité de recherche UR12ES06, Physiologie de l'Exercice et Physiopathologie: de l'Intégré au Moléculaire "Biologie, Médecine et Santé," Faculté de Médecine de Sousse, Université de Sousse, Sousse, Tunisia.
Faculté des Sciences de Bizerte, Université de Carthage, Bizerte, Tunisia.
出版信息
Dis Markers. 2017;2017:6198526. doi: 10.1155/2017/6198526. Epub 2017 Nov 26.
AIMS
The impact of MMP-1 (-519A/G, -1607 1G/2G), MMP-3 Lys45Glu (A/G), MMP-7 -181A/G, and MMP-12 -82A/G variants and plasma MMP levels on obesity and microvascular reactivity in Tunisians.
METHODS
Our population included 202 nonobese and 168 obese subjects. Anthropometric, biochemical, and microvascular parameters were determined according to standard protocols. PCR-RFLP and ELISA were used to determine the genetic variants and levels of MMPs, respectively.
RESULTS
The MMP-3 45Glu (G) allele associates with higher anthropometric values and MMP-3 levels compared to AA genotype carriers (BMI (kg/m): 30 ± 0.51 versus 27.33 ± 0.8, = 0.004; MMP-3 levels: 7.45 (4.77-11.91) versus 5.21 (3.60-10.21) ng/ml, = 0.006). The MMP-12 -82G allele was also associated with higher BMI values when compared to subjects carrying the AA genotype (31.41 ± 0.85 versus 28.76 ± 0.43, < 0.001). Individuals carrying the MMP-3 45G or MMP-12 -82G variants were also associated with a higher risk for severe forms of obesity (MMP-3: OR = 1.9, = 0.002; MMP-12: OR = 2.63, = 0.003). Similarly, the MMP-7 -181G allele was associated with a higher MMP-7 level and an increased risk for morbid obesity when compared to AA genotype carriers (0.32 (0.31-0.60) versus 0.18 (0.17-0.24) ng/ml, = 0.01; OR = 1.67, = 0.02, resp.).
CONCLUSION
MMP-3, MMP-7, and MMP-12 polymorphisms associate with obesity risk and its severity.
目的
研究基质金属蛋白酶-1（-519A/G、-1607 1G/2G）、基质金属蛋白酶-3 Lys45Glu（A/G）、基质金属蛋白酶-7-181A/G 和基质金属蛋白酶-12-82A/G 变体以及血浆基质金属蛋白酶水平对突尼斯人肥胖和微血管反应的影响。
方法
我们的研究人群包括 202 名非肥胖者和 168 名肥胖者。根据标准方案测定了人体测量学、生化和微血管参数。PCR-RFLP 和 ELISA 分别用于确定遗传变异和 MMP 水平。
结果
与 AA 基因型携带者相比，MMP-3 45Glu（G）等位基因与更高的人体测量值和 MMP-3 水平相关（BMI（kg/m）：30±0.51 与 27.33±0.8， = 0.004；MMP-3 水平：7.45（4.77-11.91）与 5.21（3.60-10.21）ng/ml， = 0.006）。与携带 AA 基因型的个体相比，MMP-12-82G 等位基因也与更高的 BMI 值相关（31.41±0.85 与 28.76±0.43， < 0.001）。携带 MMP-3 45G 或 MMP-12-82G 变体的个体也与严重肥胖的风险增加相关（MMP-3：OR=1.9， = 0.002；MMP-12：OR=2.63， = 0.003）。同样，与携带 AA 基因型的个体相比，MMP-7-181G 等位基因与更高的 MMP-7 水平和病态肥胖的风险增加相关（0.32（0.31-0.60）与 0.18（0.17-0.24）ng/ml， = 0.01；OR=1.67， = 0.02）。
结论
MMP-3、MMP-7 和 MMP-12 多态性与肥胖风险及其严重程度相关。
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