Eser B, Eser O, Yuksel Y, Aksit H, Karavelioglu E, Tosun M, Sekerci Z
Department of Medical Genetics, Faculty of Medicine, Balıkesir University, Balıkesir, Turkey
Department of Neurosurgery, Faculty of Medicine, Balıkesir University, Balıkesir, Turkey.
Genet Mol Res. 2016 Sep 9;15(3):gmr8669. doi: 10.4238/gmr.15038669.
The aim of this study was to identify the possible correlation between polymorphisms in matrix metalloproteinase (MMP)-1 and MMP-3 and their corresponding protein levels in disc tissues obtained from patients with lumbar disc herniation (LDH) using biochemical and immunohistochemical analyses. Blood and disc samples were obtained from 100 patients with LDH who underwent a lumbar microdiscectomy. Based on the radiological degeneration, the patients were diagnosed with grade 2, 3, or 4 LDH. MMP-1 -1607 1G/2G and MMP-3 -1171 5A/6A were analyzed by real-time polymerase chain reaction. The expressions of MMP-1 and MMP- 3 were detected by biochemical and immunohistochemical analyses. We found no association between the MMP-1 polymorphism and disc degeneration and MMP-1 expression. However, patients expressing the 6A/6A and 5A/6A alleles of MMP-3 -11715A/6A showed higher MMP-3 expression, compared to those expressing the 5A/5A genotype. Additionally, the radiological degeneration grades were correlated with the histological degeneration scoring. Protein levels and immunopositive cell rates of MMP-1 and MMP-3 were associated with disc degeneration grades. Moreover, the MMP-1 and MMP-3 expression and the histological and radiological scores were positively correlated and the MMP-3 -11715A/6A polymorphism was associated with MMP-3 expression in herniated disc tissues. This study is the first to investigate polymorphisms in MMP-1 and MMP-3, as well as their corresponding protein expressions. We also quantified an association between the radiological degeneration grades and MMP-1 and MMP- 3 expression. Further genomic studies on MMPs could focus on the utilization of MMP-1 and MMP-3 as markers for the prevention and treatment of this disease.
本研究旨在通过生化和免疫组织化学分析，确定腰椎间盘突出症（LDH）患者椎间盘组织中基质金属蛋白酶（MMP）-1和MMP-3基因多态性与其相应蛋白水平之间的可能相关性。从100例行腰椎显微椎间盘切除术的LDH患者中获取血液和椎间盘样本。根据影像学退变情况，将患者诊断为2级、3级或4级LDH。通过实时聚合酶链反应分析MMP-1 -1607 1G/2G和MMP-3 -1171 5A/6A。通过生化和免疫组织化学分析检测MMP-1和MMP-3的表达。我们发现MMP-1基因多态性与椎间盘退变及MMP-1表达之间无关联。然而，与表达5A/5A基因型的患者相比，表达MMP-3 -1171 5A/6A的6A/6A和5A/6A等位基因的患者显示出更高的MMP-3表达。此外，影像学退变分级与组织学退变评分相关。MMP-1和MMP-3的蛋白水平及免疫阳性细胞率与椎间盘退变分级相关。而且，MMP-1和MMP-3的表达与组织学和影像学评分呈正相关，MMP-3 -1171 5A/6A基因多态性与突出椎间盘组织中的MMP-3表达相关。本研究首次调查了MMP-1和MMP-3的基因多态性及其相应的蛋白表达。我们还量化了影像学退变分级与MMP-1和MMP-3表达之间的关联。关于MMPs的进一步基因组研究可聚焦于将MMP-1和MMP-3用作该疾病预防和治疗的标志物。
