Choi Sae Byeol, Han Hyung Joon, Kim Wan Bae, Song Tae Jin, Choi Sang Yong
Department of Surgery, Korea University Guro Hospital, Korea University College of Medicine, Seoul, Korea.
Department of Surgery, Korea University Guro Hospital, 80, Guro-dong, Guro-gu, Seoul152-703, Korea, Tel: +82-2-2626-3076.
Open Med (Wars). 2017 Dec 9;12:430-439. doi: 10.1515/med-2017-0061. eCollection 2017.
The aim of this study was to investigate the clinicopathological and immunohistochemical (including VEGF, Akt, HSP70, and HSP20 expression) factors that affect the overall and disease-free survival of HCC patients following surgical resection.
234 patients with HCC following surgical resection were enrolled. Clinicopathological and survival data were analyzed, and immunohistochemical staining was performed on tissue microarray sections using the anti-VEGF, anti-Akt, anti-HSP70, and anti-HSP27 antibodies.
The 3- and 5-year overall survival rates were 86.5 and 81.54%, respectively. Multivariate analysis revealed that VEGF expression (P = 0.017, HR = 2.573) and T stage (P < 0.001, HR = 4.953) were independent prognostic factors for overall survival. Immunohistochemical staining showed that the expression of Akt, HSP70, and HSP27 did not affect the overall survival rate. The 3- and 5-year disease-free survival rates were 58.2 and 49.4%, respectively. Compared to the VEGF(-)/(+) group, the VEGF(++)/(+++) group demonstrated significantly higher proportion of patients with AFP levels > 400 ng/mL, capsule invasion, and microvascular invasion.
VEGF overexpression was associated with capsule invasion, microvascular invasion, and a poor overall survival rate.
本研究旨在调查影响肝癌患者手术切除后总生存期和无病生存期的临床病理及免疫组化（包括VEGF、Akt、HSP70和HSP20表达）因素。
纳入234例手术切除后的肝癌患者。分析临床病理和生存数据，并使用抗VEGF、抗Akt、抗HSP70和抗HSP27抗体对组织芯片切片进行免疫组化染色。
3年和5年总生存率分别为86.5%和81.54%。多因素分析显示，VEGF表达（P = 0.017，HR = 2.573）和T分期（P < 0.001，HR = 4.953）是总生存期的独立预后因素。免疫组化染色显示，Akt、HSP70和HSP27的表达不影响总生存率。3年和5年无病生存率分别为58.2%和49.4%。与VEGF(-)/(+)组相比，VEGF(++)/(+++)组中AFP水平>400 ng/mL、包膜侵犯和微血管侵犯的患者比例显著更高。
VEGF过表达与包膜侵犯、微血管侵犯及较差的总生存率相关。
