Carl R. Woese Institute of Genomic Biology, University of Illinois at Urbana-Champaign, 1207 West Gregory Drive, Urbana, IL, 61801, USA.
Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
Cell Tissue Res. 2018 Jun;372(3):507-522. doi: 10.1007/s00441-017-2764-z. Epub 2018 Jan 9.
Advances in stem cell biology and materials science have provided a basis for developing tissue engineering methods to repair muscle injury. Among stem cell populations with potential to aid muscle repair, adipose-derived mesenchymal stem cells (ASC) hold great promise. To evaluate the possibility of using porcine ASC for muscle regeneration studies, we co-cultured porcine ASC with murine CC myoblasts. These experiments demonstrated that porcine ASC display significant myogenic potential. Co-culture of ASC expressing green fluorescent protein (GFP) with CC cells resulted in GFP myotube formation, indicating fusion of ASC with myoblasts to form myotubes. The presence of porcine lamin A/C positive nuclei in myotubes and RTqPCR analysis of porcine myogenin and desmin expression confirmed that myotube nuclei derived from ASC contribute to muscle gene expression. Co-culturing GFPASC with porcine satellite cells demonstrated enhanced myogenic capability of ASC, as the percentage of labeled myotubes increased compared to mouse co-cultures. Enhancing myogenic potential of ASC through soluble factor treatment or expansion of ASC with innate myogenic capacity should allow for their therapeutic use to regenerate muscle tissue lost to disease or injury.
干细胞生物学和材料科学的进展为开发组织工程方法修复肌肉损伤提供了基础。在具有潜在辅助肌肉修复能力的干细胞群体中，脂肪来源的间充质干细胞（ASC）具有巨大的应用潜力。为了评估猪 ASC 用于肌肉再生研究的可能性，我们将猪 ASC 与鼠 C2C12 成肌细胞共培养。这些实验表明，猪 ASC 具有显著的成肌潜力。共培养表达绿色荧光蛋白（GFP）的 ASC 与 C2C12 细胞导致 GFP 肌管形成，表明 ASC 与成肌细胞融合形成肌管。在肌管中存在猪层粘连蛋白 A/C 阳性核，以及对猪肌生成素和结蛋白表达的 RTqPCR 分析证实，肌管核来源于 ASC，有助于肌肉基因表达。将 GFPASC 与猪卫星细胞共培养证明了 ASC 的成肌能力增强，与鼠共培养相比，标记的肌管百分比增加。通过可溶性因子处理增强 ASC 的成肌潜能，或通过固有成肌能力扩增 ASC，应该允许其用于治疗因疾病或损伤而失去的肌肉组织。
