Bril H, De Ruiter H, Hussaarts-Odijk L M, Bianchi A T, Benner R
Transplantation. 1985 Oct;40(4):417-21.
Delayed-type hypersensitivity (DTH) against histocompatibility (H) antigens in mice, which normally arises after s.c. immunization, can be prevented by i.v. preimmunization with irradiated spleen cells carrying the relevant H antigens. We have previously shown that during the first week after i.v. preimmunization the nonresponsiveness is due to suppressor T lymphocytes. The induced state of nonresponsiveness, however, is long-lasting. In this study we investigated whether this long-lasting state of nonresponsiveness of DTH is associated with suppressor T lymphocytes or is caused by inactivation of the relevant clones of alloreactive T cells. This was done by parabiosis of nonresponsive and naive mice and by transfer of thoracic duct lymphocytes from nonresponsive mice, harvested at various intervals after the i.v. immunization. At a long interval after the i.v. immunization, the state of nonresponsiveness could still be transferred to syngeneic naive mice by parabiosis, as well as by transfer of thoracic duct lymphocytes. Selective elimination of the T cells from the latter by treatment with anti-Thy-1.2 plus complement prevented the transfer of the state of nonresponsiveness, indicating that suppressor T cells were involved.
小鼠对组织相容性（H）抗原的迟发型超敏反应（DTH）通常在皮下免疫后出现，而静脉注射携带相关H抗原的经辐照脾细胞进行预免疫可预防这种反应。我们之前已经表明，在静脉注射预免疫后的第一周内，无反应性是由于抑制性T淋巴细胞所致。然而，诱导产生的无反应状态是持久的。在本研究中，我们调查了DTH这种持久的无反应状态是与抑制性T淋巴细胞有关，还是由同种反应性T细胞的相关克隆失活引起的。这是通过将无反应小鼠与未致敏小鼠联体共生，以及转移静脉注射免疫后不同时间采集的无反应小鼠的胸导管淋巴细胞来完成的。在静脉注射免疫后的较长时间间隔，无反应状态仍可通过联体共生以及胸导管淋巴细胞的转移传递给同基因未致敏小鼠。用抗Thy-1.2加补体处理后从后者中选择性清除T细胞可阻止无反应状态的传递，表明抑制性T细胞参与其中。
