Department of Neurosurgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, China.
Department of Neurosurgery, Children's Hospital of Nanjing Medical University, Nanjing, China.
J Cell Physiol. 2018 Sep;233(9):6822-6831. doi: 10.1002/jcp.26432. Epub 2018 Mar 25.
LncRNA HOX transcript antisense intergenic RNA (HOTAIR) has been shown to play prominent roles in tumorigenesis. However, its precise molecular mechanism in glioma has not been completely elucidated. In this study, we found that HOTAIR was aberrantly up-regulated in glioma tissues and was negatively correlated with miR-126-5p expression. Next, we determined that HOTAIR promote glioma progression by sponging miR-126-5p. Subsequently, glutaminase (GLS) was confirmed to be a direct target of miR-126-5p using bioinformatics software and a luciferase reporter assay. Moreover, HOTAIR could modulate GLS expression by functioning as a competing endogenous RNA (ceRNA) for miR-126-5p. Taken together, our study clarified that the HOTAIR/miR-126/GLS pathway is involved in glioma progression and may potentially serve as a target for glioma therapy.
长链非编码 RNA HOX 转录反义基因间 RNA（HOTAIR）已被证明在肿瘤发生中发挥重要作用。然而，其在神经胶质瘤中的精确分子机制尚未完全阐明。在这项研究中，我们发现 HOTAIR 在神经胶质瘤组织中异常上调，并且与 miR-126-5p 表达呈负相关。接下来，我们确定 HOTAIR 通过海绵 miR-126-5p 促进神经胶质瘤的进展。随后，通过生物信息学软件和荧光素酶报告基因实验证实谷氨酰胺酶（GLS）是 miR-126-5p 的直接靶标。此外，HOTAIR 可以通过作为 miR-126-5p 的竞争性内源性 RNA（ceRNA）来调节 GLS 的表达。总之，我们的研究阐明了 HOTAIR/miR-126/GLS 通路参与神经胶质瘤的进展，并且可能作为神经胶质瘤治疗的靶点。
