Ciogli Alessia, Ismail Omar H, Mazzoccanti Giulia, Villani Claudio, Gasparrini Francesco
Dipartimento di Chimica e Tecnologie del Farmaco, "Sapienza" Università di Roma, Rome, Italy.
J Sep Sci. 2018 Mar;41(6):1307-1318. doi: 10.1002/jssc.201701406. Epub 2018 Feb 2.
The ever-increasing need for enantiomerically pure chiral compounds has greatly expanded the number of enantioselective separation methods available for the precise and accurate measurements of the enantiomeric purity. The introduction of chiral stationary phases for liquid chromatography in the last decades has revolutionized the routine methods to determine enantiomeric purity of chiral drugs, agrochemicals, fragrances, and in general of organic and organometallic compounds. In recent years, additional efforts have been placed on faster, enantioselective analytical methods capable to fulfill the high throughput requirements of modern screening procedures. Efforts in this field, capitalizing on improved chromatographic particle technology and dedicated instrumentation, have led to highly efficient separations that are routinely completed on the seconds time scale. An overview of the recent achievements in the field of ultra-high-resolution chromatography on column packed with chiral stationary phases, both based on sub-2 μm fully porous and sub-3 μm superficially porous particles, will be given, with an emphasis on very recent studies on ultrafast chiral separations.
对映体纯的手性化合物的需求不断增加，极大地扩展了用于精确测量对映体纯度的对映选择性分离方法的数量。过去几十年中液相色谱手性固定相的引入，彻底改变了测定手性药物、农用化学品、香料以及一般有机和有机金属化合物对映体纯度的常规方法。近年来，人们进一步致力于开发能够满足现代筛选程序高通量要求的更快的对映选择性分析方法。该领域利用改进的色谱颗粒技术和专用仪器所做的努力，已实现了高效分离，通常能在数秒时间内完成。本文将概述在手性固定相填充柱上进行的超高分辩率色谱领域的最新成果，这些成果基于亚2微米全多孔和亚3微米表面多孔颗粒，重点介绍超快手性分离的最新研究。
