Kiel J W, Riedel G L, DiResta G R, Shepherd A P
Am J Physiol. 1985 Oct;249(4 Pt 1):G539-45. doi: 10.1152/ajpgi.1985.249.4.G539.
To determine the feasibility of measuring gastric mucosal blood flow by laser-Doppler velocimetry (LDV), we utilized two LDV flowmeters to monitor blood flow in mucosa and serosa of chambered canine stomach. In isolated, nonautoregulating gastric segments vasodilated with isoproterenol, LDV mucosal and muscularis blood flows were both linearly related to total electromagnetic blood flow during step increases in perfusion pressure. To assess the depth of the LDV measurement, we recorded reactive hyperemia following arterial occlusion. Reactive hyperemia was frequently registered in the mucosa but rarely in muscularis. Placing a layer of nonperfused mucosa-submucosa between the probe and the perfused mucosa abolished the resting LDV mucosal flow signal and attenuated the recording of peak hyperemia by 85%. Furthermore, intra-arterial infusions of both adenosine and isoproterenol frequently increased LDV mucosal flow and decreased LDV muscularis flow, although total flow was consistently increased. These findings indicate that our LDV instruments yield linear, superficial measurements of gastric blood flow in either mucosa or muscularis. Although calibration in absolute units remains to be achieved, our results demonstrate that LDV is a practical means of studying the gastric mucosal microcirculation.
为了确定用激光多普勒测速仪（LDV）测量胃黏膜血流的可行性，我们使用两台LDV流量计来监测犬腔室胃黏膜和浆膜的血流。在使用异丙肾上腺素扩张血管的离体、无自身调节功能的胃段中，在灌注压逐步升高时，LDV测量的黏膜和肌层血流均与总电磁血流呈线性相关。为了评估LDV测量的深度，我们记录了动脉闭塞后的反应性充血情况。反应性充血在黏膜中经常出现，但在肌层中很少出现。在探头与灌注黏膜之间放置一层未灌注的黏膜 - 黏膜下层，可消除LDV记录的黏膜静息血流信号，并使充血峰值记录衰减85%。此外，动脉内注射腺苷和异丙肾上腺素均经常增加LDV测量的黏膜血流并降低LDV测量的肌层血流，尽管总血流持续增加。这些发现表明，我们的LDV仪器能够对胃黏膜或肌层血流进行线性的、浅表的测量。尽管仍有待实现以绝对单位进行校准，但我们的结果表明，LDV是研究胃黏膜微循环的一种实用方法。
