Reith W, Forsting M, Weber J, Stingele R, Hacke W, Sartor K
Department of Neuroradiology, University of Heidelberg, Federal Republic of Germany.
Acta Neurochir (Wien). 1994;131(1-2):151-6. doi: 10.1007/BF01401466.
Monitoring cerebral blood flow during focal ischaemia and reperfusion with established techniques such as hydrogen clearance and autoradiography is difficult. Laser Doppler flowmetry is a new technique, it allows one to continuously measure blood flow in small tissue samples. The objective of this study was to compare laser Doppler flowmetry with hydrogen clearance using a new single fiber probe to obtain measurements in deep brain structures and then to show the temporal profile of cerebral blood flow during focal ischaemia and after reperfusion. First, the single fiber laser Doppler method was compared with the hydrogen clearance method in ten Wistar rats. Second, focal cerebral ischaemia was induced in fifteen Wistar rats using a model of middle cerebral artery occlusion based on the intravascular insertion of a nylon suture; reperfusion occurred after withdrawal of the suture. The laser Doppler probe was placed in the lateral caudatoputamen, and local cerebral blood flow was measured continuously before and during occlusion as well as after reperfusion. The relative blood flow values obtained by the laser Doppler method and the hydrogen clearance method showed a good correlation (r = 0.76) and a linear relationship. A rapid decrease in laser Doppler flowmetry to 42 +/- 16% of former baseline values was seen with occlusion of the middle cerebral artery; during occlusion cerebral blood flow remained at this level. Reperfusion resulted in a heterogeneous pattern of cerebral blood flow as laser Doppler flowmetry values ranged from 25% to 134% of baseline values. The effects of middle cerebral artery occlusion and reperfusion on cerebral blood flow can be monitored on-line with laser Doppler flowmetry.(ABSTRACT TRUNCATED AT 250 WORDS)
使用氢清除法和放射自显影术等成熟技术监测局灶性缺血和再灌注期间的脑血流量很困难。激光多普勒血流仪是一项新技术，它能让人连续测量小组织样本中的血流量。本研究的目的是使用一种新型单纤维探头，将激光多普勒血流仪与氢清除法进行比较，以获取深部脑结构的测量值，进而展示局灶性缺血期间及再灌注后脑血流量的时间变化情况。首先，在十只Wistar大鼠中，将单纤维激光多普勒法与氢清除法进行比较。其次，在十五只Wistar大鼠中，采用基于尼龙缝线血管内插入的大脑中动脉闭塞模型诱导局灶性脑缺血；取出缝线后实现再灌注。将激光多普勒探头置于外侧尾状核壳核，在闭塞前、闭塞期间以及再灌注后连续测量局部脑血流量。激光多普勒法和氢清除法获得的相对血流量值显示出良好的相关性（r = 0.76）和线性关系。大脑中动脉闭塞时，激光多普勒血流仪测量值迅速降至先前基线值的42±16%；闭塞期间脑血流量维持在这一水平。再灌注导致脑血流量呈现异质性模式，激光多普勒血流仪测量值在基线值的25%至134%之间。大脑中动脉闭塞和再灌注对脑血流量的影响可用激光多普勒血流仪进行在线监测。（摘要截选至250词）
