van der Graaff M, Hofman P H, Breimer D D, Vermeulen N P, Knabe J, Schamber L
Biomed Mass Spectrom. 1985 Sep;12(9):464-9. doi: 10.1002/bms.1200120904.
A stereospecific synthesis of N1-(2H3)-labelled R(-)-hexobarbital is described. A sensitive and rapid selected ion monitoring assay procedure for pseudoracemic hexobarbital, consisting of equal amounts of S(+)-hexobarbital (1a) and (2H3)-R(-)-hexobarbital (1b) in 100 microliters blood samples of rats was developed. Both the parent enantiomers in blood and three major metabolites excreted in urine were quantified. An application of the method in rats is described, and the results are compared to previously obtained data of separately administered enantiomers.
描述了N1-(2H3)标记的R(-)-己巴比妥的立体定向合成。开发了一种灵敏且快速的伪外消旋己巴比妥选择离子监测测定方法，该方法用于测定大鼠100微升血液样本中等量的S(+)-己巴比妥(1a)和(2H3)-R(-)-己巴比妥(1b)。对血液中的母体对映体和尿液中排泄的三种主要代谢物进行了定量分析。描述了该方法在大鼠中的应用，并将结果与先前分别给予对映体所获得的数据进行了比较。
