Vermeulen A M, Rosseel M T, Belpaire F M
Heymans Institute of Pharmacology, University of Ghent Medical School, Belgium.
J Chromatogr. 1991 Jul 5;567(2):472-9. doi: 10.1016/0378-4347(91)80154-5.
The enantiospecific determination of R- and S-hexobarbital in rat plasma is described. The method involves liquid-liquid extraction of racemic hexobarbital from plasma, separation of the underivatized enantiomers by high-performance liquid chromatography on an alpha 1-acid glycoprotein column and ultraviolet detection. The mobile phase consists of a phosphate buffer (pH 5.4) containing 0.4% 2-propanol as organic modifier. An alpha 1-acid glycoprotein guard column is used to increase the lifetime of the analytical column. Heptabarbital is the achiral internal standard. With detection limits of ca. 0.05 microgram/ml for both R- and S-hexobarbital, the assay is suitable for pharmacokinetic studies of the enantiomers in rats.
本文描述了大鼠血浆中R-和S-己巴比妥的对映体特异性测定方法。该方法包括从血浆中液-液萃取外消旋己巴比妥，在α1-酸性糖蛋白柱上通过高效液相色谱法分离未衍生化的对映体并进行紫外检测。流动相由含有0.4%异丙醇作为有机改性剂的磷酸盐缓冲液（pH 5.4）组成。使用α1-酸性糖蛋白保护柱以延长分析柱的使用寿命。庚巴比妥为非手性内标。R-和S-己巴比妥的检测限约为0.05微克/毫升，该测定法适用于大鼠体内对映体的药代动力学研究。
