Lin Da-Cen, Lin Jia-Bing, Chen Zhou, Chen Rong, Wan Chun-Yu, Lin Shao-Wei, Ruan Qi-Shuang, Li Huang-Yuan, Wu Si-Ying
Department of Epidemiology and Health Statistics, the Key Laboratory of Environment and Health among Universities and Colleges in Fujian, School of Public Health, Fujian Medical University, Minhou County, Fuzhou, Fujian, China.
Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine, Fujian, China.
J Geriatr Cardiol. 2017 Nov;14(11):688-695. doi: 10.11909/j.issn.1671-5411.2017.11.004.
To evaluate the effects of environmental factors and microRNAs (miRNAs) (miR-126, miR-143, and miR-145) on the risk of coronary heart disease (CHD).
A frequency-matched case-control study (450 patients, 450 controls) was conducted from April 2014 to December 2016 in Fuzhou City, China. Environmental factors were investigated using a self-administered questionnaire, and the expression levels of miR-126, miR-143, and miR-145 were determined by quantitative real-time Polymerase Chain Reaction (PCR) in peripheral blood mononuclear cells. Unconditional logistic regression models were used for statistical evaluation.
Alcohol consumption, high-salt diets, high-intensity work, and lack of physical activity were significantly associated with increased CHD risk, whereas light diet was significantly associated with decreased risk. MiR-126, miR-143, and miR-145 were highly expressed in the CHD group compared with the control group. After adjustment for other environmental factors, unconditional logistic regression results revealed that miR-126, miR-143, and depression were the independent risk factors of CHD, and light diet was the independent protective factor of CHD.
Our data suggest that a family history of CHD, anxiety, and alcohol consumption was significantly associated with increased CHD risk, whereas light diet was significantly associated with decreased risk. Furthermore, miR-126 and miR-143 in combination with several risk factors, could play a joint role in the development of CHD. Therefore, it is necessary to manage patients with CHD in all directions and multiple level.
评估环境因素和微小RNA（miRNA）（miR-126、miR-143和miR-145）对冠心病（CHD）风险的影响。
2014年4月至2016年12月在中国福州市进行了一项频率匹配的病例对照研究（450例患者，450例对照）。使用自填问卷调查环境因素，并通过定量实时聚合酶链反应（PCR）测定外周血单个核细胞中miR-126、miR-143和miR-145的表达水平。采用无条件逻辑回归模型进行统计评估。
饮酒、高盐饮食、高强度工作和缺乏体育活动与冠心病风险增加显著相关，而清淡饮食与风险降低显著相关。与对照组相比，CHD组中miR-126、miR-143和miR-145高表达。在调整其他环境因素后，无条件逻辑回归结果显示，miR-126、miR-143和抑郁是冠心病的独立危险因素，清淡饮食是冠心病的独立保护因素。
我们的数据表明，冠心病家族史、焦虑和饮酒与冠心病风险增加显著相关，而清淡饮食与风险降低显著相关。此外，miR-126和miR-143与多种危险因素共同作用，可能在冠心病的发生发展中发挥联合作用。因此，有必要对冠心病患者进行全方位、多层次的管理。
