聚二乙酰基纳米纤维作为新型 siRNA 载体用于体外和体内递送。
Polydiacetylenic nanofibers as new siRNA vehicles for in vitro and in vivo delivery.
机构信息
V-SAT Laboratory, Vectors: Synthesis and Therapeutic Applications, Labex Medalis, CAMB UMR7199 CNRS-Université de Strasbourg, Faculty of Pharmacy, Illkirch, France.
出版信息
Nanoscale. 2018 Jan 25;10(4):1587-1590. doi: 10.1039/c7nr09202d.
Polydiacetylenic nanofibers (PDA-Nfs) obtained by photopolymerization of surfactant 1 were optimized for intracellular delivery of small interfering RNAs (siRNAs). PDA-Nfs/siRNA complexes efficiently silenced the oncogene Lim-1 in the renal cancer cells 786-O in vitro. Intraperitoneal injection of PDA-Nfs/siLim1 downregulated Lim-1 in subcutaneous tumor xenografts obtained with 786-O cells in nude mice. Thus, PDA-Nfs represent an innovative system for in vivo delivery of siRNAs.
通过光聚合表面活性剂 1 得到的聚二乙酰亚胺纳米纤维（PDA-Nfs）被优化用于小分子干扰 RNA（siRNA）的细胞内传递。PDA-Nfs/siRNA 复合物在体外有效沉默了肾癌细胞 786-O 中的致癌基因 Lim-1。用 PDA-Nfs/siLim1 腹腔注射，可下调裸鼠皮下肿瘤异种移植中由 786-O 细胞获得的 Lim-1。因此，PDA-Nfs 代表了一种用于体内递送 siRNA 的创新系统。
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