Yin Shuai, Wang Zhao, Xu Jia-Qi, Xu Wen-Mei, Yuan Pan-Pan, Cui Heng-Lin
School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People's Republic of China.
Arch Microbiol. 2015 Dec;197(10):1159-64. doi: 10.1007/s00203-015-1159-3. Epub 2015 Oct 5.
A halophilic archaeal strain, YJ-18-S1(T), was isolated from Yangjiang marine solar saltern, Guangxi Province, China. Cells were pleomorphic, stained Gram-negative and formed red-pigmented colonies on agar plates. Strain YJ-18-S1(T) was able to grow at 20-55 °C (optimum 37 °C), at 0.9-4.8 M NaCl (optimum 2.6 M NaCl), at 0.005-1.0 M MgCl2 (optimum 0.3 MgCl2) and at pH 5.5-8.5 (optimum pH 7.0). The cells were lysed in distilled water, and the minimal NaCl concentration to prevent cell lysis was found to be 5 % (w/v). The major polar lipids of the strain were phosphatidic acid, phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and sulfated mannosyl glucosyl diether. The 16S rRNA gene and rpoB' gene of strain YJ-18-S1(T) were phylogenetically related to the corresponding genes of Halorubrum members (94.3-98.0 and 86.7-96.1 % similarities, respectively). The DNA G+C content of strain YJ-18-S1(T) was 66.2 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain YJ-18-S1(T) (=CGMCC 1.12554(T) = JCM 30030(T)) represents a new species of Halorubrum, for which the name Halorubrum rutilum sp. nov. is proposed.
从中国广西阳江海洋太阳盐场分离出一株嗜盐古菌，命名为YJ-18-S1(T)。细胞形态多样，革兰氏染色阴性，在琼脂平板上形成红色菌落。菌株YJ-18-S1(T)能够在20-55°C（最适温度37°C）、0.9-4.8 M NaCl（最适浓度2.6 M NaCl）、0.005-1.0 M MgCl2（最适浓度0.3 M MgCl2）以及pH 5.5-8.5（最适pH 7.0）的条件下生长。细胞在蒸馏水中会裂解，防止细胞裂解的最低NaCl浓度为5%（w/v）。该菌株的主要极性脂类为磷脂酸、磷脂酰甘油、磷脂酰甘油磷酸甲酯、磷脂酰甘油硫酸酯和硫酸化甘露糖基葡糖二醚。菌株YJ-18-S1(T)的16S rRNA基因和rpoB'基因在系统发育上与嗜盐红菌属成员的相应基因相关（相似性分别为94.3-98.0%和86.7-96.1%）。菌株YJ-18-S1(T)的DNA G+C含量为66.2 mol%。其表型、化学分类和系统发育特性表明，菌株YJ-18-S1(T)（=CGMCC 1.12554(T) = JCM 30030(T)）代表嗜盐红菌属的一个新物种，为此提出新种名为嗜盐红菌（Halorubrum rutilum sp. nov.）。
