Kharel Sitaula Ranju, Janani M K, Madhavan H N, Biswas Jyotirmay
Department of Ophthalmology, Maharajgunj Medical Campus, B. P. Koirala Lions Centre for Ophthalmic Studies, Institute of Medicine, Tribhuvan University, Maharajgunj, Kathmandu, Nepal.
Sankara Nethralaya Referral Laboratory, Chennai, India.
J Ophthalmic Inflamm Infect. 2018 Jan 10;8(1):2. doi: 10.1186/s12348-017-0144-1.
Polymerase chain reaction (PCR) analysis is an important tool in the diagnosis of infectious uveitis. A retrospective, interventional study of PCR analysis of ocular fluid in suspected infectious uveitis cases between January 2014 to July 2016 was done. Nested, real-time and broad range PCR was performed for detection of the genome of Mycobacterium tuberculosis, herpes virus family, Chikungunya virus, Toxoplasma gondii, fungus, eubacterium and propionibacterium acne.
Total of 100 cases included, mean age was 39.2 ± 15.4 years. Uveitis was unilateral in 82% and granulomatous in 40%. Mean visual acuity at the initial visit and final visit was 0.73 logMar and 0.63 logMar respectively. PCR analysis confirmed the clinical diagnosis in 70.1% patients. The sensitivity, specificity, positive predictive value and negative predictive value of PCR analysis was 90.2%, 93.9%, 93.9% and 90.2% respectively. The quantitative value of real-time M. tb. Positive PCR ranged from 32c/ml to 2722 c/ml.
PCR assay is an accurate technique with high sensitivity and specificity to diagnose the DNA genome in infectious uveitis.
聚合酶链反应（PCR）分析是感染性葡萄膜炎诊断中的一项重要工具。对2014年1月至2016年7月间疑似感染性葡萄膜炎病例的眼内液进行PCR分析的一项回顾性干预研究。采用巢式、实时和广谱PCR检测结核分枝杆菌、疱疹病毒科、基孔肯雅病毒、弓形虫、真菌、真细菌和痤疮丙酸杆菌的基因组。
共纳入100例病例，平均年龄为39.2±15.4岁。82%的葡萄膜炎为单侧，40%为肉芽肿性。初诊和末次就诊时的平均视力分别为0.73 logMar和0.63 logMar。PCR分析在70.1%的患者中证实了临床诊断。PCR分析的敏感性、特异性、阳性预测值和阴性预测值分别为90.2%、93.9%、93.9%和90.2%。实时结核分枝杆菌阳性PCR的定量值范围为32c/ml至2722 c/ml。
PCR检测是一种诊断感染性葡萄膜炎中DNA基因组的准确技术，具有高敏感性和特异性。
