使用波长调制拉曼光谱法检测表型耐药结核分枝杆菌

Detecting Phenotypically Resistant Mycobacterium tuberculosis Using Wavelength Modulated Raman Spectroscopy.

作者信息

Baron Vincent O, Chen Mingzhou, Clark Simon O, Williams Ann, Dholakia Kishan, Gillespie Stephen H

机构信息

School of Medicine, University of St Andrews, St Andrews, UK.

SUPA, School of Physics and Astronomy, University of St Andrews, St Andrews, UK.

出版信息

Methods Mol Biol. 2018;1736:41-50. doi: 10.1007/978-1-4939-7638-6_4.

DOI:10.1007/978-1-4939-7638-6_4

PMID:29322457

Abstract

Raman spectroscopy is a non-destructive and label-free technique. Wavelength modulated Raman (WMR) spectroscopy was applied to investigate Mycobacterium tuberculosis cell state, lipid rich (LR) and lipid poor (LP). Compared to LP cells, LR cells can be up to 40 times more resistant to key antibiotic regimens. Using this methodology single lipid rich (LR) from lipid poor (LP) bacteria can be differentiated with both high sensitivity and specificity. It can also be used to investigate experimentally infected frozen tissue sections where both cell types can be differentiated. This methodology could be utilized to study the phenotype of mycobacterial cells in other tissues.

摘要

拉曼光谱是一种无损且无需标记的技术。波长调制拉曼（WMR）光谱被用于研究结核分枝杆菌的细胞状态，即富含脂质（LR）和脂质缺乏（LP）状态。与LP细胞相比，LR细胞对关键抗生素治疗方案的耐药性可能高达40倍。使用这种方法，可以高灵敏度和特异性地区分来自脂质缺乏（LP）细菌的单个富含脂质（LR）细胞。它还可用于研究实验感染的冷冻组织切片，其中两种细胞类型都可以被区分。这种方法可用于研究其他组织中分枝杆菌细胞的表型。