Wildner Leticia Muraro, Gould Katherine A, Waddell Simon J
Universidade Federal de Santa Catarina, Florianópolis, Santa Catarina, Brazil.
Department of Global Health and Infection, Brighton and Sussex Medical School, University of Sussex, Brighton, UK.
Methods Mol Biol. 2018;1736:117-128. doi: 10.1007/978-1-4939-7638-6_11.
The emergence of drug resistance threatens to destroy tuberculosis control programs worldwide, with resistance to all first-line drugs and most second-line drugs detected. Drug tolerance (or phenotypic drug resistance) is also likely to be clinically relevant over the 6-month long standard treatment for drug-sensitive tuberculosis. Transcriptional profiling the response of Mycobacterium tuberculosis to antimicrobial drugs offers a novel interpretation of drug efficacy and mycobacterial drug-susceptibility that likely varies in dynamic microenvironments, such as the lung. This chapter describes the noninvasive sampling of tuberculous sputa and techniques for mRNA profiling M. tb bacilli during patient therapy to characterize real-world drug actions.
耐药性的出现有可能破坏全球结核病控制项目，目前已检测到对所有一线药物和大多数二线药物的耐药情况。在针对药物敏感型结核病的长达6个月的标准治疗期间，药物耐受性（或表型耐药性）在临床上也可能具有相关性。对结核分枝杆菌对抗菌药物的反应进行转录谱分析，为药物疗效和分枝杆菌药敏性提供了一种新的解释，而这在动态微环境（如肺部）中可能会有所不同。本章介绍了结核痰液的非侵入性采样以及在患者治疗期间对结核杆菌进行mRNA谱分析的技术，以表征实际的药物作用。
