Vallarino M
Gen Comp Endocrinol. 1985 Oct;60(1):63-9. doi: 10.1016/0016-6480(85)90293-x.
By immunocytochemical methods the present study describes beta-endorphin-like immunoreactive substance in the brain of Boops boops. Beta-Endorphin-like neurons and fibers were detected in both dorsal and ventral components of the preoptic nucleus and in the nucleus lateralis tuberis. This localization has been discussed in relation to the presence, in the same area, of a well-defined neurosecretory system involved in hypophysial regulation. A beta-endorphin-like immunoreaction was also detected in the Purkinje cells and in processes within the cerebellum molecular layer. Although this last finding remains enigmatic it may suggest a neuromodulatory activity for cerebellum beta-endorphin-like substance. No immunoreaction was observed when the specific antiserum was absorbed with corresponding antigen and with beta-LPH. These tests led to the conclusion that the immunostaining reaction might correspond to a beta-endorphin- or lipotropin-like reaction. Further, the present results show the phylogenetic antiquity of a beta-endorphinergic or lipotropinergic system in the brain, with a stable evolutionary history as a hypophysial regulatory factor or neuromodulatory agent.
本研究通过免疫细胞化学方法描述了黄斑海鲷大脑中的β-内啡肽样免疫反应物质。在视前核的背侧和腹侧部分以及外侧结节核中检测到了β-内啡肽样神经元和纤维。已结合同一区域中参与垂体调节的明确神经分泌系统的存在对这一定位进行了讨论。在浦肯野细胞和小脑分子层内的突起中也检测到了β-内啡肽样免疫反应。尽管这一最新发现仍不明朗，但它可能表明小脑β-内啡肽样物质具有神经调节活性。当特异性抗血清与相应抗原及β-LPH一起吸收时，未观察到免疫反应。这些试验得出结论，免疫染色反应可能对应于β-内啡肽或促脂解素样反应。此外，目前的结果表明，大脑中β-内啡肽能或促脂解素能系统具有系统发育上的古老性，作为垂体调节因子或神经调节剂具有稳定的进化史。
