State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, MUST, China.
FEBS Lett. 2018 Feb;592(3):380-393. doi: 10.1002/1873-3468.12968. Epub 2018 Jan 23.
Accumulated evidence in the last decade implies that regulation of metabolism by p53 represents a reviving mechanism vital to prevent tumorigenesis. To gain a more in-depth understanding of metabolic regulation by baseline levels of p53, we employed both metabolomics and transcriptomics analysis with human colon cancer cell-line HCT116 depleted of p53. Metabolomics analyses with UPLC/quadrupole time-of-flight mass spectrometry identified 283 significantly changed metabolites including 138 important metabolites. Transcriptomics analysis with microarray revealed 1317 differentially expressed genes. By integrated analysis of both omics data, we found nucleotides metabolism and sulfur-related metabolism are of great importance. Our study provided a pilot comprehensive view of the metabolism regulated by p53 and suggests several potential p53 targets in metabolism for further study.
过去十年的研究证据表明，p53 对代谢的调控代表了一种重要的复苏机制，对于预防肿瘤发生至关重要。为了更深入地了解 p53 基线水平对代谢的调控，我们采用代谢组学和转录组学分析方法，研究了 p53 缺失的人结肠癌细胞系 HCT116。采用 UPLC/四极杆飞行时间质谱联用技术的代谢组学分析鉴定出 283 种显著变化的代谢物，包括 138 种重要代谢物。采用微阵列的转录组学分析揭示了 1317 个差异表达基因。通过对这两种组学数据的综合分析，我们发现核苷酸代谢和硫相关代谢非常重要。我们的研究为 p53 调控的代谢提供了一个初步的综合视图，并为代谢中的几个潜在 p53 靶点提供了进一步研究的建议。
