Glukhovets Boris I, Glukhovets N G, Belitchenko N V, Sosunova O A
Vopr Virusol. 2016;61(5):219-21.
Congenital herpes infection belongs to the category of actual problems of Perinatal Medicine. Pathological diagnosis of this disease is not effective in the routine method of autopsy studies without virological research. Objective. Determination of the value of the fluorescent antibody technique in the diagnosis of congenital herpes infection of the stillborn is a promising approach to medical diagnosis. subjects and methods. In 96 cases of stillbirth immunofluorescent identification of herpes simplex virus types 1 and 2 and cytomegalovirus in the placenta and internal organs (brain, heart, lungs, and liver) was implemented. The findings were compared with the results of a complete histological examination of the heart, including its rhythmogenic centers. Results. The herpes viruses were found in 51 observations (53.1%). Among them, HSV-1 were found in 16 observations (16.7%), HSV-2, in 19 (19.7%), CMV, in 16 (16.7%). In 34 stillbirths (35.8%) the pathological signs of herpetic atrial myocarditis were observed, which were regarded as the cause of death. Conclusion. The use of the fluorescent antibody technique in the autopsy practice is an effective way of diagnosis of intrauterine infection caused by the herpes simplex virus and cytomegalovirus.
先天性疱疹感染属于围产医学的实际问题范畴。在没有病毒学研究的常规尸检方法中，这种疾病的病理诊断并不有效。目的。确定荧光抗体技术在诊断死产儿先天性疱疹感染中的价值是医学诊断的一种有前景的方法。对象和方法。对96例死产儿的胎盘及内脏器官（脑、心、肺和肝）进行1型和2型单纯疱疹病毒以及巨细胞病毒的免疫荧光鉴定。将结果与心脏包括其节律发生中心的完整组织学检查结果进行比较。结果。在51例观察中发现了疱疹病毒（53.1%）。其中，16例观察中发现了HSV - 1（16.7%），19例中发现了HSV - 2（19.7%），16例中发现了CMV（16.7%）。在34例死产儿（35.8%）中观察到疱疹性心房心肌炎的病理征象，其被视为死亡原因。结论。在尸检实践中使用荧光抗体技术是诊断由单纯疱疹病毒和巨细胞病毒引起的宫内感染的有效方法。
