Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501, Japan.
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501, Japan.
Bioresour Technol. 2018 Mar;252:157-164. doi: 10.1016/j.biortech.2017.12.100. Epub 2017 Dec 30.
We investigated the use of low concentrations of butanol (<40%, all v/v) as an organosolv pretreatment to fractionate lignocellulosic biomass into cellulose, hemicellulose, and lignin. The pretreatment conditions were optimized for sorghum bagasse by focusing on four parameters: butanol concentration, sulfuric acid concentration, pretreatment temperature, and pretreatment time. A butanol concentration of 25% or higher together with 0.5% or higher acid was effective for removing lignin while retaining most of the cellulose in the solid fraction. The highest cellulose (84.9%) and low lignin (15.3%) content were obtained after pretreatment at 200 °C for 60 min. Thus, pretreatment comprising 25% butanol, 0.5% acid, 200 °C, and 60 min process time was considered optimal. Enzymatic saccharification and fermentation by Saccharomyces cerevisiae produced 61.9 g/L ethanol from 200 g/L solid fraction obtained following pretreatment, and 10.2 g/L ethanol was obtained from the liquid fraction by xylose-utilizing S. cerevisiae following membrane nanofiltration to remove butanol.
我们研究了使用低浓度丁醇（<40%，均为体积/体积）作为有机溶剂预处理，将木质纤维素生物质分离为纤维素、半纤维素和木质素。通过关注四个参数：丁醇浓度、硫酸浓度、预处理温度和预处理时间，对高粱秸秆进行了优化。丁醇浓度为 25%或更高，同时酸浓度为 0.5%或更高，可有效去除木质素，同时保留固体部分中的大部分纤维素。在 200°C 下预处理 60 分钟后，获得了最高的纤维素（84.9%）和最低的木质素（15.3%）含量。因此，25%丁醇、0.5%酸、200°C 和 60 分钟的处理时间被认为是最佳的预处理条件。通过 Saccharomyces cerevisiae 的酶解和发酵，从预处理后获得的 200g/L 固体部分中生产出 61.9g/L 的乙醇，通过膜纳滤去除丁醇后，利用木糖的 S. cerevisiae 从液体部分中获得 10.2g/L 的乙醇。
