Helmholtz-Zentrum Dresden-Rossendorf, Institute of Resource Ecology, Bautzner Landstraße 400, D-01328 Dresden, Germany.
Helmholtz-Zentrum Dresden-Rossendorf, Institute of Resource Ecology, Bautzner Landstraße 400, D-01328 Dresden, Germany.
J Hazard Mater. 2018 Apr 5;347:233-241. doi: 10.1016/j.jhazmat.2017.12.030. Epub 2017 Dec 28.
The contamination of the environment by U may affect plant life and consequently may have an impact on animal and human health. The present work describes U(VI) sequestration by Acidovorax facilis using a multidisciplinary approach combining wet chemistry, transmission electron microscopy, and spectroscopy methods (e.g. cryo-time resolved laser-induced fluorescence spectroscopy, extended X-ray absorption fine structure spectroscopy, and in-situ attenuated total reflection Fourier transform infrared spectroscopy). This bacterial strain is widely distributed in nature including U-contaminated sites. In kinetic batch experiments cells of A. facilis were contacted for 5 min to 48 h with 0.1 mM U(VI). The results show that the local coordination of U species associated with the cells depends upon time contact. U is bound mainly to phosphate groups of lipopolysaccharide (LPS) at the outer membrane within the first hour. And, that both, phosphoryl and carboxyl functionality groups of LPS and peptidoglycan of A. facilis cells may effectuate the removal of high U amounts from solution at 24-48 h of incubation. It is clearly demonstrated that A. facilis may play an important role in predicting the transport behaviour of U in the environment and that the results will contribute to the improvement of bioremediation methods of U-contaminated sites.
U 对环境的污染可能会影响植物的生命，从而可能对动物和人类的健康产生影响。本工作采用多种学科方法，包括湿法化学、透射电子显微镜和光谱学方法（例如，低温时间分辨激光诱导荧光光谱学、扩展 X 射线吸收精细结构光谱学和原位衰减全反射傅里叶变换红外光谱学），描述了 Acidovorax facilis 对 U(VI)的螯合作用。这种细菌广泛分布于自然界中，包括 U 污染的地点。在动力学批量实验中，将 A. facilis 的细胞与 0.1mM U(VI)接触 5 分钟至 48 小时。结果表明，与细胞相关的 U 物种的局部配位取决于接触时间。在最初的一个小时内，U 主要与 LPS 的磷酸基团结合，位于外膜内。并且，LPS 和 A. facilis 细胞的肽聚糖的磷酸基和羧基官能团可能在孵育 24-48 小时时从溶液中去除大量的 U。显然，A. facilis 可能在预测 U 在环境中的迁移行为方面发挥重要作用，并且结果将有助于改进 U 污染地点的生物修复方法。
