Lin Zhong, Zhen Zhen, Ren Lei, Yang Jiewen, Luo Chunling, Zhong Laiyuan, Hu Hanqiao, Liang Yanqiu, Li Yongtao, Zhang Dayi
College of Agriculture, Guangdong Ocean University, Zhanjiang 524088, PR China.
College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, PR China.
Chemosphere. 2018 Apr;196:467-475. doi: 10.1016/j.chemosphere.2017.12.177. Epub 2017 Dec 29.
Vermicomposting is an effective and environmentally friendly approach for eliminating soil organic contamination. Atrazine is one of the most commonly applied triazinic herbicides and frequently detected in agricultural soils. This study investigated the roles and mechanisms of two earthworm species (epigeic Eisenia foetida and endogeic Amynthas robustus) in microbial degradation of atrazine. Both earthworms accelerated atrazine degradation performance from 39.0% in sterile soils to 94.9%-95.7%, via neutralizing soil pH, consuming soil humus, altering bacterial community structure, enriching indigenous atrazine degraders and excreting the intestinal atrazine-degrading bacteria. Rhodoplanes and Kaistobacter were identified as soil indigenous degraders for atrazine mineralization and stimulated by both earthworm species. A. robustus excreted the intestinal Cupriavidus and Pseudomonas, whereas Flavobacterium was released by E. foetida. This study provides a comprehensive understanding of the distinct effects of two earthworm species on soil microbial community and atrazine degradation, offering technical supports to apply vermicomposting in effective soil bioremediation.
蚯蚓堆肥是一种有效且环保的消除土壤有机污染的方法。阿特拉津是最常用的三嗪类除草剂之一，在农业土壤中经常被检测到。本研究调查了两种蚯蚓（表栖的赤子爱胜蚓和内栖的粗壮环毛蚓）在阿特拉津微生物降解中的作用和机制。两种蚯蚓都通过中和土壤pH值、消耗土壤腐殖质、改变细菌群落结构、富集本土阿特拉津降解菌以及排泄肠道阿特拉津降解菌，将阿特拉津在无菌土壤中的降解率从39.0%提高到了94.9%-95.7%。红游动菌属和海杆菌属被鉴定为土壤中阿特拉津矿化的本土降解菌，且受到两种蚯蚓的刺激。粗壮环毛蚓排泄出肠道中的贪铜菌属和假单胞菌属，而赤子爱胜蚓释放出黄杆菌属。本研究全面了解了两种蚯蚓对土壤微生物群落和阿特拉津降解的不同影响，为将蚯蚓堆肥应用于有效的土壤生物修复提供了技术支持。
