Sánchez-López Katia Berenice, De Los Santos-Ramos Francisco J, Gómez-Acata Elizabeth Selene, Luna-Guido Marco, Navarro-Noya Yendi E, Fernández-Luqueño Fabián, Dendooven Luc
Nanoscience and Nanotechnology Program, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Mexico City, Mexico.
Department of Physiology, Biophysics and Neuroscience, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Mexico City, Mexico.
PeerJ. 2019 Jul 25;7:e6939. doi: 10.7717/peerj.6939. eCollection 2019.
The amount of nanoparticles (NP), such as TiO, has increased substantially in the environment. It is still largely unknown, however, how NP might interact with earthworms and organic material and how this might affect the bacterial community structure and their functionality. Therefore, an arable soil was amended with TiO NP at 0, 150 or 300 mg kg and subjected to different treatments. Treatments were soil amended with ten earthworms ( (Savigny, 1826)) with fully developed clitellum and an average fresh mass of 0.5 to 500 g dry soil, 1.75 g tyndallized Quaker oat seeds (L.) kg, or earthworms plus oat seeds, or left unamended. The bacterial community structure was monitored throughout the incubation period. The bacterial community in the unamended soil changed over time and application of oats, earthworm and a combination of both even further, with the largest change found in the latter. Application of NP to the unamended soil and the earthworm-amended soil altered the bacterial community, but combining it by adding oats negated that effect. It was found that the application of organic material, that is, oats, reduced the effect of the NP applied to soil. However, as the organic material applied was mineralized by the soil microorganisms, the effect of NP increased again over time.
环境中纳米颗粒（NP）的数量，如二氧化钛纳米颗粒，已大幅增加。然而，纳米颗粒如何与蚯蚓和有机物质相互作用，以及这如何影响细菌群落结构及其功能，在很大程度上仍不清楚。因此，在可耕地土壤中添加0、150或300毫克/千克的二氧化钛纳米颗粒，并进行不同处理。处理方式包括：添加十条环带发育完全、平均鲜重为0.5至500克（相对于干土）的蚯蚓（正蚓，萨维尼，1826年）、每千克土壤添加1.75克经间歇灭菌的桂格燕麦种子（燕麦）、蚯蚓加燕麦种子，或不进行添加。在整个培养期监测细菌群落结构。未添加任何物质的土壤中的细菌群落随时间变化，添加燕麦、蚯蚓以及两者组合后变化更大，其中以两者组合的变化最大。向未添加任何物质的土壤和添加蚯蚓的土壤中添加纳米颗粒会改变细菌群落，但添加燕麦后这种影响被抵消。研究发现，添加有机物质（即燕麦）会降低土壤中纳米颗粒的影响。然而，随着添加的有机物质被土壤微生物矿化，纳米颗粒的影响会随着时间再次增加。
