Lee Young-Tae, Ko Eun-Ju, Lee Youri, Kim Ki-Hye, Kim Min-Chul, Lee Yu-Na, Kang Sang-Moo
Center for Inflammation, Immunity & Infection, Institute for Biomedical Sciences, Georgia State University, Atlanta, Georgia, United States of America.
Animal and Plant Quarantine Agency, Gimcheon, Gyeongsangbukdo, Republic of Korea.
PLoS One. 2018 Jan 11;13(1):e0190868. doi: 10.1371/journal.pone.0190868. eCollection 2018.
Current influenza vaccines do not provide broad cross-protection. Here, we report that intranasal vaccination with virus-like particles containing the highly conserved multiple ectodomains of matrix protein 2 (M2e5x VLP) of influenza virus induces broad cross-protection by M2-specific humoral and cellular immune responses. M2e5x VLP intranasal vaccination prevented severe weight loss, attenuated inflammatory cytokines and cellular infiltrates, and lowered viral loads, and induced germinal center phenotypic B and plasma cells. In addition, depletion studies demonstrate the protective roles of CD4 and CD8 T cells induced by M2e5x VLP intranasal vaccination. Thus, this study provides evidence that mucosal delivery of M2e5x VLP vaccine provides cross-protection by inducing humoral and cellular immune responses.
目前的流感疫苗不能提供广泛的交叉保护。在此，我们报告称，用含有流感病毒基质蛋白2高度保守的多个胞外域（M2e5x VLP）的病毒样颗粒进行鼻内接种，可通过M2特异性体液免疫和细胞免疫反应诱导广泛的交叉保护。M2e5x VLP鼻内接种可防止严重体重减轻，减轻炎性细胞因子和细胞浸润，并降低病毒载量，还可诱导生发中心表型的B细胞和浆细胞。此外，耗竭研究证明了M2e5x VLP鼻内接种诱导的CD4和CD8 T细胞的保护作用。因此，本研究提供了证据，表明M2e5x VLP疫苗的黏膜递送通过诱导体液免疫和细胞免疫反应提供交叉保护。
