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联合单磷酰脂质 A 和聚肌苷酸胞苷酸对流感疫苗抗原特异性免疫应答和保护效力的佐剂作用。

Adjuvant effects of combination monophosphoryl lipid A and poly I:C on antigen-specific immune responses and protective efficacy of influenza vaccines.

机构信息

Interdisciplinary Graduate Program in Advanced Convergence Technology and Science, Jeju National University, Jeju, 63243, Republic of Korea.

Center for Inflammation, Immunity and Infection, Institute for Biomedical Sciences, Georgia State University, Atlanta, GA, 30303, USA.

出版信息

Sci Rep. 2023 Jul 28;13(1):12231. doi: 10.1038/s41598-023-39210-6.

DOI:10.1038/s41598-023-39210-6
PMID:37507413
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10382554/
Abstract

Toll-like receptor (TLR) agonists improve vaccine immunogenicity and efficacy, but they are currently unlicensed as adjuvants in influenza vaccines. This study aimed to investigate whether a combination of monophosphoryl lipid A (MPL, a TLR4 agonist) and polyriboinosinic polyribocytidylic acid (poly I:C, a TLR3 agonist) can enhance the protective efficacy of an inactivated A/Puerto Rico/8/1934 (A/PR8) H1N1 influenza vaccine against homologous influenza infection and minimize illness outcomes. Results showed that combination MPL and poly I:C adjuvanted influenza vaccination increased the production of antigen-specific antibodies, decreased the levels of cytokines and cellular infiltrates at the infection sites, and induced significant memory T and B cell responses in mice. The results of this study suggest that the combination of MPL and poly I:C can be developed into a possible adjuvant for enhancing the efficacy of influenza vaccines.

摘要

Toll 样受体 (TLR) 激动剂可提高疫苗的免疫原性和疗效，但它们目前尚未获得流感疫苗佐剂的许可。本研究旨在探讨单磷酰脂质 A (MPL，TLR4 激动剂) 和聚肌苷酸聚胞苷酸 (poly I:C，TLR3 激动剂) 的联合使用是否可以增强灭活 A/Puerto Rico/8/1934 (A/PR8) H1N1 流感疫苗对同源流感感染的保护效力，并最小化疾病结局。结果表明，联合使用 MPL 和 poly I:C 佐剂的流感疫苗接种可增加抗原特异性抗体的产生，降低感染部位细胞因子和细胞浸润的水平，并诱导小鼠产生显著的记忆 T 和 B 细胞反应。本研究结果表明，MPL 和 poly I:C 的联合使用可以开发成一种增强流感疫苗效力的潜在佐剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63ea/10382554/f415429e3636/41598_2023_39210_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63ea/10382554/542b71f1baa5/41598_2023_39210_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63ea/10382554/2e7235b94bcf/41598_2023_39210_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63ea/10382554/d361bb75b75b/41598_2023_39210_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63ea/10382554/5d7754534bf4/41598_2023_39210_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63ea/10382554/6cd849b21ddc/41598_2023_39210_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63ea/10382554/dd9dd13b0f52/41598_2023_39210_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63ea/10382554/f415429e3636/41598_2023_39210_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63ea/10382554/542b71f1baa5/41598_2023_39210_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63ea/10382554/2e7235b94bcf/41598_2023_39210_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63ea/10382554/d361bb75b75b/41598_2023_39210_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63ea/10382554/5d7754534bf4/41598_2023_39210_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63ea/10382554/6cd849b21ddc/41598_2023_39210_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63ea/10382554/dd9dd13b0f52/41598_2023_39210_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63ea/10382554/f415429e3636/41598_2023_39210_Fig7_HTML.jpg

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