Ansari Afsana, Wang Chunlian, Wang Jian, Wang Fujun, Liu Piqing, Gao Ying, Tang Yongchao, Zhao Kaijun
National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agriculture Sciences, Beijing, China.
Crop Institute of Ningxia Academy of Agriculture and Forestry Sciences, Yingchuan, China.
Front Plant Sci. 2017 Dec 13;8:2132. doi: 10.3389/fpls.2017.02132. eCollection 2017.
Rice is a crop feeding half of the world's population. With the continuous raise of yield potential via genetic improvement, rice breeding has entered an era where multiple genes conferring complex traits must be efficiently manipulated to increase rice yield further. Recurrent selection is a sound strategy for manipulating multiple genes and it has been successfully performed in allogamous crops. However, the difficulties in emasculation and hand pollination had obstructed efficient use of recurrent selection in autogamous rice. Here, we report development of the dwarf male-sterile rice that can facilitate recurrent selection in rice breeding. We adopted RNAi technology to synergistically regulate rice plant height and male fertility to create the dwarf male-sterile rice. The RNAi construct pTCK-EGGE, targeting the and genes, was constructed and used to transform rice via -mediated transformation. The transgenic T0 plants showing largely reduced plant height and complete male-sterile phenotypes were designated as the dwarf male-sterile plants. Progenies of the dwarf male-sterile plants were obtained by pollinating them with pollens from the wild-type. In the T1 and T2 populations, half of the plants were still dwarf male-sterile; the other half displayed normal plant height and male fertility which were designated as tall and male-fertile plants. The tall and male-fertile plants are transgene-free and can be self-pollinated to generate new varieties. Since emasculation and hand pollination for dwarf male-sterile rice plants is no longer needed, the dwarf male-sterile rice can be used to perform recurrent selection in rice. A dwarf male-sterile rice-based recurrent selection model has been proposed.
水稻是养活世界一半人口的作物。随着通过基因改良不断提高产量潜力，水稻育种已进入一个必须有效操控多个赋予复杂性状的基因以进一步提高水稻产量的时代。轮回选择是操控多个基因的合理策略，并且已在异花授粉作物中成功实施。然而，去雄和人工授粉的困难阻碍了轮回选择在自花授粉水稻中的有效应用。在此，我们报道了可促进水稻育种中轮回选择的矮秆雄性不育水稻的培育。我们采用RNA干扰技术协同调控水稻株高和雄性育性以创制矮秆雄性不育水稻。构建了靶向[具体基因名称缺失]基因的RNA干扰载体pTCK - EGGE，并通过[具体介导方式缺失]介导转化用于转化水稻。表现出株高大幅降低和完全雄性不育表型的转基因T0植株被指定为矮秆雄性不育植株。用野生型花粉给矮秆雄性不育植株授粉获得其后代。在T1和T2群体中，一半植株仍为矮秆雄性不育；另一半表现出正常株高和雄性育性，被指定为高秆可育植株。高秆可育植株不含转基因，可自花授粉以培育新品种。由于不再需要对矮秆雄性不育水稻植株进行去雄和人工授粉，矮秆雄性不育水稻可用于水稻的轮回选择。已提出了基于矮秆雄性不育水稻的轮回选择模型。
