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利用烟草属植物作为替代宿主研究葫芦科细菌性果斑病菌（Acidovorax citrulli）的致病性。

Nicotiana species as surrogate host for studying the pathogenicity of Acidovorax citrulli, the causal agent of bacterial fruit blotch of cucurbits.

机构信息

School of Plant and Environmental Sciences, Virginia Tech, Blacksburg, VA, USA.

Department of Plant Pathology and Microbiology, The Hebrew University of Jerusalem, Rehovot, Israel.

出版信息

Mol Plant Pathol. 2019 Jun;20(6):800-814. doi: 10.1111/mpp.12792. Epub 2019 Apr 1.

DOI:10.1111/mpp.12792
PMID:30938096
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6637898/
Abstract

Bacterial fruit blotch (BFB) caused by Acidovorax citrulli is one of the most important bacterial diseases of cucurbits worldwide. However, the mechanisms associated with A. citrulli pathogenicity and genetics of host resistance have not been extensively investigated. We idenitfied Nicotiana benthamiana and Nicotiana tabacum as surrogate hosts for studying A. citrulli pathogenicity and non-host resistance triggered by type III secreted (T3S) effectors. Two A. citrulli strains, M6 and AAC00-1, that represent the two major groups amongst A. citrulli populations, induced disease symptoms on N. benthamiana, but triggered a hypersensitive response (HR) on N. tabacum plants. Transient expression of 19 T3S effectors from A. citrulli in N. benthamiana leaves revealed that three effectors, Aave_1548, Aave_2708, and Aave_2166, trigger water-soaking-like cell death in N. benthamiana. Aave_1548 knockout mutants of M6 and AAC00-1 displayed reduced virulence on N. benthamiana and melon (Cucumis melo L.). Transient expression of Aave_1548 and Aave_2166 effectors triggered a non-host HR in N. tabacum, which was dependent on the functionality of the immune signalling component, NtSGT1. Hence, employing Nicotiana species as surrogate hosts for studying A. citrulli pathogenicity may help characterize the function of A. citrulli T3S effectors and facilitate the development of new strategies for BFB management.

摘要

细菌性果斑病（BFB）由酸噬果胶杆菌（Acidovorax citrulli）引起，是全球葫芦科作物最重要的细菌性病害之一。然而，该病原菌的致病机制以及寄主抗性的遗传学基础尚未得到广泛研究。本研究将黄花烟（Nicotiana benthamiana）和普通烟（Nicotiana tabacum）鉴定为研究酸噬果胶杆菌致病性和由 III 型分泌效应子（T3S）触发的非寄主抗性的替代寄主。代表酸噬果胶杆菌种群中两个主要群体的两个菌株（M6 和 AAC00-1）在黄花烟上诱导出病害症状，但在普通烟上引发了过敏反应（HR）。在黄花烟叶片中瞬时表达 19 个酸噬果胶杆菌的 T3S 效应子后发现，3 个效应子 Aave_1548、Aave_2708 和 Aave_2166 在黄花烟叶片中引发水渍样细胞死亡。M6 和 AAC00-1 的 Aave_1548 敲除突变体在黄花烟和甜瓜（Cucumis melo L.）上的毒力降低。瞬时表达 Aave_1548 和 Aave_2166 效应子在非寄主普通烟上引发非寄主 HR，该 HR 依赖于免疫信号成分 NtSGT1 的功能。因此，利用黄花烟和普通烟作为替代寄主来研究酸噬果胶杆菌的致病性可能有助于表征酸噬果胶杆菌 T3S 效应子的功能，并为 BFB 管理开发新策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97fd/6637898/d2303d828adc/MPP-20-800-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97fd/6637898/bbf363a24373/MPP-20-800-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97fd/6637898/301ca44912a5/MPP-20-800-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97fd/6637898/b891572ce3c3/MPP-20-800-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97fd/6637898/d2303d828adc/MPP-20-800-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97fd/6637898/bbf363a24373/MPP-20-800-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97fd/6637898/301ca44912a5/MPP-20-800-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97fd/6637898/b891572ce3c3/MPP-20-800-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97fd/6637898/d2303d828adc/MPP-20-800-g004.jpg

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