Rasoulinezhad Saeed, Bozorgmehrifard Mohammad Hassan, Hosseini Hossein, Sheikhi Nariman, Charkhkar Saeed
Department of Poultry and Obstetrics, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Clinical Science, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Alborz, Iran.
Vet Res Forum. 2017;8(4):293-298. Epub 2017 Dec 15.
(MG) is economically important pathogen of poultry causes airsacculitis and frequently infraorbital sinusitis in turkeys. Infections may remain without clinical signs, but they can make birds susceptible to secondary infections. This study was carried out for molecular detection and phylogenetic analysis of MG infections in commercial and backyard turkey flocks in some parts of Iran. A total number of 600 swab samples were collected from 18 commercial and 31 backyard turkey flocks. The PCR technique was performed for detecting 16S rRNA gene in the samples. Positive sample were subjected for sequencing of mgc2 gene. The results showed that 48.38% of backyard and 16.66% of commercial farms were positive for MG. These findings suggested the presence of MG in the commercial and backyard turkeys' farms of Iran. The molecular analysis indicated high sequence similarity between some Iranian turkeys isolates with Indian and Pakistanian MG isolates. Furthermore, substitutions of MG nucleic acids and correlated amino acids sequences may lead to some antigenic modifications.
支原体（MG）是一种对家禽具有经济重要性的病原体，可导致火鸡气囊炎，并经常引发眶下窦炎。感染可能没有临床症状，但会使鸟类易受继发感染。本研究旨在对伊朗部分地区商业和后院火鸡群中的MG感染进行分子检测和系统发育分析。共从18个商业火鸡群和31个后院火鸡群中采集了600份拭子样本。采用PCR技术检测样本中的16S rRNA基因。对阳性样本进行mgc2基因测序。结果显示，后院火鸡群中48.38%呈MG阳性，商业养殖场中16.66%呈MG阳性。这些发现表明伊朗商业和后院火鸡养殖场中存在MG。分子分析表明，一些伊朗火鸡分离株与印度和巴基斯坦的MG分离株具有高度的序列相似性。此外，MG核酸和相关氨基酸序列的替换可能导致一些抗原性修饰。
