Pattanshetty Smita, Kotrashetti Vijayalakshmi S, Bhat Kishore, Nayak Ramakant S, Somannavar Pradeep, Pujar Madhu
Department of Oral Pathology and Microbiology, Maratha Mandal's Nathajirao G Halgekar's Institute of Dental Sciences, Karnataka, India.
Department of Conservative Dentistry and Endodontics, Maratha Mandal's Nathajirao G Halgekar's Institute of Dental Sciences, Karnataka, India.
J Investig Clin Dent. 2018 May;9(2):e12312. doi: 10.1111/jicd.12312. Epub 2018 Jan 11.
The aim of the present study was to investigate the presence of selective anaerobic microorganisms in primary root canal infections of symptomatic and asymptomatic non-vital teeth with periapical pathosis using multiplex polymerase chain reaction.
A total of 100 root canal samples (50 from symptomatic and 50 from asymptomatic teeth) were obtained from patients with primary endodontic infections. DNA extracted from the samples was amplified by using specific primers for the 16S rRNA gene of each bacterium, and semiquantification was done to analyze the prevalence of microorganisms and their correlation to clinical features.
Treponema denticola (T. denticola) was present in 21 (42%) and 29 (58%) samples in the symptomatic and asymptomatic groups, respectively. Tannerella forsythia, Porphyromonas gingivalis (P. gingivalis), and Fusobacterium nucleatum (F. nucleatum) were significantly high (P < .05) in the symptomatic group, whereas Prevotella intermedia was significantly high (P < .05) in the asymptomatic group. The mean counts of T. denticola and F. nucleatum were significantly high (P < .05) in the symptomatic group. For symptoms, P. gingivalis, T. denticola, and F. nucleatum were significantly associated with clinical features.
Significant differences exist in the bacterial composition between asymptomatic and symptomatic primary endodontic infections. As well as presence of pathogens, other factors, such as the phenotypic trait of bacteria and interactions among bacterial members, might play a determining role in the pathogenicity of primary endodontic infections.
本研究旨在采用多重聚合酶链反应，调查有根尖周病变的有症状和无症状非活髓牙的原发性根管感染中选择性厌氧微生物的存在情况。
从患有原发性牙髓感染的患者中获取了总共100个根管样本（50个来自有症状牙齿，50个来自无症状牙齿）。使用针对每种细菌16S rRNA基因的特异性引物对从样本中提取的DNA进行扩增，并进行半定量分析以分析微生物的流行情况及其与临床特征的相关性。
在有症状组和无症状组中，分别有21个（42%）和29个（58%）样本中存在具核梭杆菌（T. denticola）。在有症状组中，福赛坦纳菌、牙龈卟啉单胞菌（P. gingivalis）和具核梭杆菌（F. nucleatum）的检出率显著较高（P < 0.05），而在无症状组中，中间普氏菌的检出率显著较高（P < 0.05）。在有症状组中，具核梭杆菌和T. denticola的平均计数显著较高（P < 0.05）。对于症状，牙龈卟啉单胞菌、具核梭杆菌和T. denticola与临床特征显著相关。
无症状和有症状的原发性牙髓感染之间的细菌组成存在显著差异。除了病原体的存在外，其他因素，如细菌的表型特征和细菌成员之间的相互作用，可能在原发性牙髓感染的致病性中起决定性作用。
