Krajcovicová-Kudlácková M, Dibák O
Physiol Bohemoslov. 1985;34(4):339-50.
Male Wistar rats aged 75 and 150 days were given high fat diet (36.5 weight % and 30 weight % fat) over a period of 14 days. The growth (PER, NPR) and utilization (NPU, LPU) parameters of protein biological value and liver phosphoenolpyruvate carboxykinase (PEPCK) activity were determined. In another experiment, the time dependence of liver gluconeogenesis enzyme (PEPCK and fructose-1,6-diphosphatase /FDP-ase/) and transaminase (alanine and aspartate aminotransferase /ALT, AST/) activities during 24 days' administration of the diet were determined. A 14 days' high fat intake had a negative effect on protein utilization in the organism of 75- and 150-day-old animals, which was more pronounced in the younger age group (a bigger drop in net protein utilization /NPU/ and greater stimulation of PEPCK activity). In 150-day-old animals the negative effect of a high fat intake was already manifested on the 6th to 10th day of the diet to the same degree as in the younger animals on the 14th day, as seen from the increase in all the enzyme activities. The paper presents findings on differences in the degree of the negative effect of a high fat intake on protein utilization with reference to age.
给75日龄和150日龄的雄性Wistar大鼠喂食高脂饮食（脂肪含量分别为36.5重量%和30重量%），持续14天。测定了蛋白质生物学价值的生长（蛋白质功效比值、净蛋白质比值）和利用（净蛋白质利用率、蛋白质真消化率）参数以及肝脏磷酸烯醇式丙酮酸羧激酶（PEPCK）活性。在另一项实验中，测定了在24天喂食期间肝脏糖异生酶（PEPCK和果糖-1,6-二磷酸酶/FDP-ase/）和转氨酶（丙氨酸转氨酶和天冬氨酸转氨酶/ALT、AST/）活性的时间依赖性。14天的高脂摄入对75日龄和150日龄动物机体的蛋白质利用有负面影响，在较年轻的年龄组中更为明显（净蛋白质利用率/NPU/下降幅度更大，PEPCK活性受到更大刺激）。在150日龄的动物中，从所有酶活性的增加可以看出，高脂摄入的负面影响在喂食的第6至10天就已经表现出来，与较年轻动物在第14天的程度相同。本文介绍了关于高脂摄入对蛋白质利用的负面影响程度随年龄差异的研究结果。
