Krajcovicová M, Dibák O
Physiol Bohemoslov. 1980;29(3):233-42.
The effect of 14 days' intake of mounting quantities of fats and energy on a constant optimum utilizable amount of dietary protein was studied in weaned rats weighing 60 g. Growth (PER, NPR) and utilization (NPU--body, LPU--liver) parameters of biological protein value were determined, supplemented by a study of the course of the antithetical processes of gluconeogenesis and glycolysis, of the pentose cycle, the citric acid cycle and of transamination processes in the animals' liver. According to the maximum PER, NPR, NPU and LPU values, a 14 days' intake of 59.2 g fats and 4.518 MJ, corresponding to a diet containing 10% protein, 30% fat and 2.274 MJ, had the best effect on the optimum utilizable amount of casein (21 g/14 days). A diet containing 35% and 40% fat stimulated gluconeogenesis, followed by a transamination process and inhibition of glycolysis, the citric acid cycle and lipogenesis (depression of pentose cycle activity and a decrease in the amount of body fat), and simultaneously reduced the values of all four parameters of protein biological value. The results demonstrate that very best diet for newly weaned rats is one containing 10% high quality protein, 30% fat and 2.274 MJ.
在体重60克的断奶大鼠中，研究了连续14天摄入逐渐增加的脂肪和能量对恒定最佳可利用量膳食蛋白质的影响。测定了生物蛋白质价值的生长参数（蛋白质功效比值、净蛋白质比值）和利用参数（净蛋白质利用率——身体、肝脏净蛋白质利用率），并对动物肝脏中糖异生和糖酵解、戊糖循环、柠檬酸循环及转氨过程等相反过程的进程进行了研究。根据最大蛋白质功效比值、净蛋白质比值、净蛋白质利用率和肝脏净蛋白质利用率的值，连续14天摄入59.2克脂肪和4.518兆焦耳能量，相当于一种含10%蛋白质、30%脂肪和2.274兆焦耳能量的日粮，对酪蛋白的最佳可利用量（21克/14天）具有最佳效果。含35%和40%脂肪的日粮刺激糖异生，随后是转氨过程，并抑制糖酵解、柠檬酸循环和脂肪生成（戊糖循环活性降低和体脂量减少），同时降低了蛋白质生物价值的所有四个参数的值。结果表明，刚断奶大鼠的最佳日粮是含10%优质蛋白质、30%脂肪和2.274兆焦耳能量的日粮。
